We have developed a system for the in vitro transcription of specific genes in rooster liver chromatin by endogenous RNA polymerase II that maintains the specificity of transcription in vivo. Radioactive transcripts synthesized in vitro were identified and quantitated by hybridization to a vast excess of cloned cDNA. The cDNA preparations employed corresponded to vitellogenin mRNA, the synthesi...

We have developed a system for the in vitro transcription of specific genes in rooster liver chromatin by endogenous RNA polymerase II that maintains the specificity of transcription in uiuo. Radioactive transcripts synthesized in vitro were identified and quantitated by hybridization to a vast excess of cloned cDNA. The cDNA preparations employed corresponded to vitellogenin mRNA, the synthesi...

We report initial studies on estrogen-mediated regulation of egg yolk protein synthesis in the rooster. Egg yolk proteins are normally synthesized as a large precursor, vitellogenin, in the liver of the laying hen; roosters synthesize vitellogenin only when treated with estrogen. Polysomal RNA from the liver of estrogen-treated roosters was translated in a reticulocyte cell-free system, and the...

this research was carried out to investigate the effects of various levels of dietary organic selenium supplementation (0, 0.1,  0.2 and  0.3 mg/kg) on reproductive traits of rooster. semen was collected using abdominal massage and samples were mixed together. sperm characteristics including percentage of motile, viable and abnormal sperms were assessed. this experiment was carried out based on...

The creatine kinase (CK) isoenzyme system is essential for motility in rooster and sea urchin sperm. In the present study, biochemical characterization as well as immunofluorescence and confocal laser microscopy with highly specific antibodies against various chicken CK isoenzymes revealed that cytosolic brain-type CK isoenzyme (B-CK) is the only CK isoenzyme in rooster seminal plasma, while th...

Phosvitin kinase, the enzyme that phosphorylates the egg yolk protein phosvitin, has been purified more than SOOO-fold from the supernatant of rooster liver, a tissue that synthesizes phosvitin only after estrogen administration. The purified enzyme prepared from the estrogen-treated liver was recovered in high yield (49%) and was very stable at 04”. It sedimented in sucrose as a single peak wi...

To determine the ME and amino acid digestibility of 5 soybean meal (SBM) samples, a precision-fed rooster assay and a chick assay were conducted. The 5 samples were cold-pressed (extruded) soybean meals or solvent-extracted (defatted) soybean meal. Of the cold-pressed varieties (unheated), there was an ultra-low trypsin SBM, a low-trypsin SBM, and a heated and unheated commodity SBM. The solven...

This study aimed to determine the effect of method giving rations during day and night on physiological response (respiratory rate, rectal temperature, heart rate) medium type roosters in postal cages best ration methods rooster cage. used a Completely Randomized Design (CRD), consisting three treatments with six replications, those were P1: 30% 70% ration; P2: 50% P3: ration. The chicken was m...

Effect of Different Levels 2, 4 Dinitrophenol and Luteolin on Semen Quality Rooster during Freezing-Thawing Process