The formation of first nodules inhibits subsequent nodulation in younger regions of alfalfa (Medicago sativa L.) roots by a feedback regulatory mechanism that controls nodule number systemically (G Caetano-Anollés, WD Bauer [1988] Planta 175: 546-557). Following inoculation with wild-type Rhizobium meliloti, almost all infections associated with cortical cell division developed into mature nodu...

Polar auxin transport is dependent on the family of PIN-formed proteins (PINs), which are membrane transporters of anionic indole-3-acetic acid (IAA(-)). It is assumed that polar auxin transport may be essential in the development and meristematic activity maintenance of Medicago truncatula (M. truncatula) root nodules. However, little is known about the involvement of specific PIN proteins in ...

We developed a rapid, inexpensive method for isolating and identifying Rhizobium leguminosarum by. phaseoli strains from bean root nodules. The method can be used for other small or medium-sized nodules such as those of clover, alfalfa and peas. With this method, one person can process 115 nodules/h. Using a simple apparatus made of plexiglass and machine bolts, we simultaneously crushed 24 sur...

Nitrogen use efficiency and life cycle of root nodules in alfalfa after different mineral fertilization and soil cultivation practices were studied. Field trial was conducted in the Institute of Forage Crops, Pleven, Bulgaria on leached chernozem subsoil type and no irrigation. The next treatments were tested: i) for fertilization as follows: N0P0K0 (control); N60P100K80 (an accepted technology...

nitrogen use efficiency and life cycle of root nodules in alfalfa after different mineral fertilization and soil cultivation practices were studied. field trial was conducted in the institute of forage crops, pleven, bulgaria on leached chernozem subsoil type and no irrigation. the next treatments were tested: i) for fertilization as follows: n0p0k0 (control); n60p100k80 (an accepted technology...

Photosynthetic data collected from Pisum sativum L. and Phaseolus vulgaris L. plants at different stages of development were related to symbiotic N(2) fixation in the root nodules. The net carbon exchange rate of each leaf varied directly with carboxylation efficiency and inversely with the CO(2) compensation point. Net carbon exchange of the lowest leaves reputed to supply fixed carbon to root...

During the course of the development of nitrogen-fixing root nodules induced by Sinorhizobium meliloti on the model plant Medicago truncatula, tubules called infection threads are cooperatively constructed to deliver the bacterial symbiont from the root surface to cells in the interior of the root and developing nodule. Three-dimensional reconstructions of infection threads inside M. truncatula...

High specific activity [(3)H]indole-3-acetic acid (IAA) was applied to the apical bud of intact pea (Pisum sativum L. cv Greenfeast) plants. Radioactivity was detected in all tissues after 24 hours. More radioactivity accumulated in the nodules than in the parent root on a fresh weight basis and more in effective (nitrogen-fixing) nodules than in ineffective nodules (which do not fix nitrogen)....