A semi-synthetic thiamine-free diet was used on weaned lambs to test the effect of a high sulfur level on the rumen, microbial activity and on the microbial production of thiamine. In vivo and in vitro kinetic studies, as well as the determination of the thiamine concentrations and thiaminase activity in the rumen, were performed during the 16 week experiment. A high sulfur level (0.6%) in the ...

BACKGROUND: Manipulation of rumen microbial population for improving animal performance is done by several methods including medicinal plants. Matricaria chamomille (chamomile) and Cichorium intybus (chicory) are two medicinal plant with antibacterial effect that there is little information about the effects of them on rumen microflora. OBJECTIVE: The present study was carried out to evaluate t...

The digestion and utilization of roughage by sheep depends on rumen digestion. The aim of this study was to evaluate the microbe quantity and fermentative efficiency of rumen supplement with molasses-urea. This experiment was conducted in 2014, eight sheep were selected and divided into two groups (a control group and a treatment group), and only the treatment group animals were supplied with m...

Capsaicin (CAP) and Yucca schidigera extract (YSE) are two types of plant extracts that can change rumen fermentation. This study was conducted to investigate whether supplementation beef cattle diets with CAP YSE for 90 days would affect fermentation microflora. Forty-five healthy Angus steers (initial body weight = 510.54 ± 41.27 kg) were divided into three groups: control (CON), CAP, YSE. Am...

A fed-batch, anaerobic culture system was developed to assess the behavior of Escherichia coli O157:H7 in a rumen-like environment. Fermentation medium consisted of either 50% (vol/vol) raw or sterile rumen fluid and 50% phosphate buffer. Additional rumen fluid was added twice per day, and samples were removed three times per day to simulate the exiting of digesta and microbes from the rumen en...

I. A study was made of the changes in electrolyte concentrations and ruminal function which resulted from the provision of 1.3 7; sodium chloride solutions instead of fresh water to sheep consuming roughage rations in chaffed and in ground pelleted forms. 2. Significantly higher osmotic pressures were observed in the rumen fluid of the sheep drinking saline water, the change being especially ma...

BACKGROUND In countries around the Baltic Sea grazing ruminants have access to and drink, surface water from lakes, rivers and in several coastal regions. The water quality of these naturally occurring reservoirs affects performance and health of livestock. In the Baltic Sea both microcystin (MC) and nodularin (NOD) occurs as cyclic peptides and have hepatotoxic effects. Although cattle obvious...

Proteolytic activity of the bovine rumen microflora was studied with azocasein as the substrate. Approximately 25% of the proteolytic activity of rumen contents was recovered in the strained rumen fluid fraction, and the balance of the activity was associated with the particulate fraction. The proportion of proteinase activity associated with particulate material decreased when the quantity of ...