Enzymatic-detergent decellularization treatments may use a combination of chemical reagents to reduce vascular tissue to sterilized scaffolds, which may be seeded with endothelial cells and implanted with a low risk of rejection. However, these chemicals may alter the mechanical properties of the native tissue and contribute to graft compliance mismatch. Uniaxial tensile data obtained from nati...

Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is commonly used to obtain high resolution separation of complex mixtures of proteins. The method initially denatures the proteins that will undergo electrophoresis. Although covalent structural features of resolved proteins can be determined with SDS-PAGE, functional properties are destroyed, including the presence of non-cov...

Since DNA fingerprinting has become widely used ' 2 3 kb in genetic analysis of different species the original method (1) has undergone modifications, e.g. in DNA extraction, Southern transfer and labeling of the DNA probe. While applying this technique for pedigree monitoring of mouse inbred strains, it was important to isolate DNA from small blood samples in order to leave the animals alive. ...

abstract digestive enzyme inhibitors are proteinacious or nonproteinacious compounds which reduce an enzyme activity through attaching to its active site and/or its substrate. nowadays, plant enzyme inhibitors are of great importance because 1) these have considerable effects on insect digestive enzymes and as a result on their development; and 2) the transgenic plants expressing them are safe....

The purpose of this research was to develop a tissue-engineered intrasynovial flexor tendon construct with the use of an acellularized flexor tendon scaffold repopulated with intrasynovial tendon cells. New Zealand white rabbit intrasynovial flexor tendons were acellularized by the following methods: high concentration NaCl + SDS, Trypsin/EDTA, Trypsin/EDTA + Triton X-100, Triton X-100, Triton ...

Monoclonal antibodies (MABs) were produced against an ethylenediaminetetraacetate (EDTA) extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgG1 and IgG2b). The EDTA extract was detected as several bands by silver staining in SDS-PAGE. In the Western blot the bands around 20 KDa reacted with a monoclonal antibody, 47B4D6, and w...

Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. Fifty isolates obtained, and thirty-three showed hydrolyzing zone on Carboxy Methyl Cellulose agar plates after Congo-Red staining. The highest specific CMCase activity was observed by C12, identified as Leclercia adecarboxylata based 16S-rRNA gene sequence analysis. SDS-PAGE of C12 ce...

Background The aim of present study is to evaluate the effect of PolyVinyl Pyrrolidone (PVP) routinly used during ICSI procedure on sperm membrane integrity, and sperm chromatin status. MaterialsAndMethods This study was carried out on 21 semen samples from the infertile men referring to Isfahan Fertility and Infertility Center. The processed semen samples were divided into two portions. One po...

Sewage water is causing a potential threat to agriculture sector due industrial effluents having heavy metals. Present investigation was carried study the role of ethylenediaminetetraacetic acid (EDTA) or aqueous extracts Hyacinth and Hedychium on soil quality growth spinach turnip plants irrigated with sewage (SW). Treatment SW resulted in an increment catalase (CAT), peroxidase (POD) polyphen...

probable use of chemical and biochemical detergents to wash polysulphone membranes using in milk ultrafiltration needs a multidisciplinary investigation. by increasing use of these membranes in food processing, washing and recovery of initial permeate flux, in order to use for a longer time from this technology, has much importance. in this research, influence of chemical detergents including e...