OBJECTIVES To assess the utility of direct plating of whole sputa onto selective media as a means of identifying antimicrobial resistance in strains of Pseudomonas aeruginosa from the sputa of patients with cystic fibrosis (CF). METHODS A total of 45 sputum samples from CF patients were cultured onto conventional culture media for isolation of P. aeruginosa and were also cultured directly ont...

background neisseria lactamica as one of the main commensal in oropharynx during the childhood is related to the induction of a natural immunity against meningococcal meningitis. also moraxella catarrhalis in oropharynx of children is a predisposing factor for otitis media infection. objectives the current study aimed to investigate the frequency of the n. lactamica, other nonpathogenic neisser...

Listeria innocua was subjected to thermal inactivation and the extent of heat-injured cells was quantified. Cultures were heated in liquid medium for different times, using temperatures in the range of 52.5 to 65.0 degrees C, and plated on Tryptic Soy Agar with 0.6% yeast extract (TSAYE) used as non-selective medium and on TSAYE plus 5% NaCl (TSAYE+NaCl) and Palcam agar with selective supplemen...

The aim of this study was to investigate the impact on numbers of using different media for the enumeration of Escherichia coli subjected to stress, and to evaluate the use of different resuscitation methods on bacterial numbers. E. coli was subjected to heat stress by exposure to 55 degrees C for 1h or to light-induced oxidative stress by exposure to artificial light for up to 8h in the presen...

numerous studies have been conducted about the role of media during a crisis. accordingly diverse functions have been defined for media while in crisis. by applying a prescriptive viewpoint to derive mass media functions, most of the studies have been the based on the opinions of experts and the opinions of the victims have rarely been taken into consideration. for the first time, we have direc...

AIMS To develop selective media for the enumeration of Botrytis cinerea. METHODS AND RESULTS Two new media, Botrytis Selective Medium (BSM) and Botrytis Spore Trap Medium (BSTM), were developed and compared with currently available media for the enumeration of B. cinerea conidia from the environment. The new Botrytis media proved advantageous over previous media because they were easier to pr...

In this researchsaccharomyces cerevisiae (baker’s yeast) was used as a cheap, readily accessible, selective, efficient, and green bio-catalyst in a chemo selective reduction of carbonyl group to hydroxyl group. In this green procedure three substrates e.g. (3-(3-nitrophenyl)aziridin-2-yl)-1-phenyl-methanone, pyruvate ester, and 2-acetyl-γ-butyrolactone were r...

Salmonellosis remains a major problematic food born zoonosis worldwide. Poultry and poultry products have been frequently incriminated as the major sources for the aetiological agent, Salmonella species. The implementation of appropriate surveillance and monitoring programme for food safety ultimately requires the examination of food for the presence of food borne pathogens. This study was aime...