In this study, we propose a complete biomass fractionation strategy where all three major biopolymers, namely, cellulose, hemicellulose, and lignin, are separated with higher efficiency purity. Sequential treatment of hybrid poplar wood using autohydrolysis (160 °C, 60 min) 1-ethyl-3-methylimidazolium acetate activation (60 3 h) resulted in significantly improved enzymatic saccharification frac...