We investigated whether lipopolysaccharide (LPS)-induced hypothermia develops in a serotype-specific manner in biotelemetered conscious rats. Two different E. coli serotypes of LPSs were injected at a dose of 250 μg/kg, ip. E. coli O55:B5 LPS elicited an initial hypothermia and subsequent fever, but E. coli O111:B4 LPS caused more potent monophasic hypothermia. Serum tumor necrosis factor (TNF)...

We investigated whether LPS-induced hypothermia develops in a serotype-specific manner in biotelemetered conscious rats. Two different Escherichia coli serotypes of LPSs were injected at a dose of 250 mug/kg ip. E. coli O55:B5 LPS elicited an initial hypothermia and subsequent fever, but E. coli O111:B4 LPS caused more potent monophasic hypothermia. Serum tumor necrosis factor (TNF)-alpha level...

Escherichia coli O111 is an emerging non-O157:H7 serotype of Shiga toxin-producing E. coli (STEC). We previously reported that outbreak and environmental, but not sporadic-case, strains of STEC O111 share a distinct aggregation phenotype (M. E. Diodati, A. H. Bates, M. B. Cooley, S. Walker, R. E. Mandrell, and M. T. Brandl, Foodborne Pathog Dis 12:235-243, 2015, http://dx.doi.org/10.1089/fpd.20...

Shiga toxin 2 (Stx2) has been reported as the main Shiga toxin associated with human disease. In addition, the Stx2 toxin type can have a profound impact on the degree of tissue damage in animal models. We have characterized the stx(2) subtype of 168 Shiga toxin-producing Escherichia coli (STEC) isolates of which 146 were derived from ovine sources (principally feces and meat) and 22 were isola...

*Corresponding author: Mailing address: Ishikawa Prefectural Institute of Public Health and Environmental Science, 1-11 Taiyohgaoka, Kanazawa 920-1154, Japan. E-mail: hokan-04@ pref.ishikawa.jp On July 7, 2004, a health center in Kanazawa City received a report of enterohemorrhagic Escherichia coli (EHEC) O111 (VT1 and 2) infection. The patient was a high school student who participated in a sc...

To the Editor: Since the first reported outbreaks of hemolytic uremic syndrome (HUS) and related conditions more than 10 years ago (1), outbreaks of HUS due to Escherichia coli O157 have been reported from many parts of the world, particularly North America and Europe. While most of these reports have incriminated the motile strains of serotype O157:H7, nonmotile serotypes (e.g., O157:H-) have ...

Few US clinical laboratories screen stool specimens for Shiga toxin-producing Escherichia coli (STEC) other than E. coli O157. An outbreak of STEC O111:H8 infections indistinguishable from E. coli O157:H7 at a youth camp highlights the need to improve non-O157 STEC surveillance. Interviews of 521 (80%) of 650 attendees revealed 55 (11%) were ill; 2 developed hemolytic-uremic syndrome. Illness w...

BACKGROUND In August 2008, the largest known US serotype 1 Escherichia coli O111 outbreak occurred in Oklahoma, causing 341 illnesses, including hemolytic uremic syndrome (HUS). HUS is not well described in non-O157 E coli outbreaks but occurs in 2% to 15% of O157 infections, predominantly among children. We examined outbreak-related hospitalizations to characterize E coli O111 illness, the HUS...

The adherence property of 70 verotoxigenicE. coli (VTEC) strains, not belonging to O157: H7 serotype (non-O157) was checked on HeLa cells adherence assay. These strains were isolated from patients with diarrhea and healthy individuals. The results obtained in this study revealed that localised adherence (LA) was manifested by 3 strains of which two belonged to serogroups O111, O127 and the thir...

BACKGROUND AND OBJECTIVES Dairy cattle have been implicated as principal reservoir of Verotoxin-Producing Escherichia coli (VTEC), with undercooked ground beef and raw milk being the major vehicles of food borne outbreaks. VTEC has been implicated as an etiological agent of individual cases and outbreaks in developed countries. This study was designed to determine the prevalence of VETEC in dia...