BACKGROUND We previously reported the development of a fully automated assay for total iron-binding capacity (TIBC) in serum, using a multipurpose automated analyzer. However, this method requires four different reagents and is thus useful only with a limited number of available analyzers. We simplified our original assay and compared the analytical performance of the modified method with that ...

BACKGROUND Melasma is a common acquired disorder characterized by symmetric, hyperpigmented patches with an irregular outline, occurring most commonly on the face. It is most prevalent among young to middle-aged women. Although iron overload affects skin pigmentation, effect of iron deficiency on skin is not clear. So, we evaluated serum iron level, ferritin and total iron binding capacity (TIB...

BACKGROUND Present methods for measuring serum total iron-binding capacity (TIBC) involve manipulation of samples or performance of two assays on each sample. We developed a direct automated assay (DTIBC) for TIBC. METHODS We added to serum a saturating amount of iron bound to an excess of chelating dye at a low pH, recorded a blank reading that represented the sum of the saturating amount of...

BACKGROUND Pregnant women constitute a high risk group for iron deficiency. Maternal iron deficiency and particularly iron deficiency anaemia may be associated with detrimental effects on maternal and infant function and particularly with a higher risk of preterm delivery and delivery of low birth weight neonates. Objective of this study was to assess and compare the iron status of normal healt...

HE DETERMINATION of serum iron (Si) and serum total iron-binding capacity (TIBC) is increasing in importance as a routine clinical measurement, as well as in ferro-kinetic studies of a research nature. Although investigations in iron metabolism are often most conveniently performed in small laboratory animals, the experimental design of such studies has been cumbersome amId severely limited by ...

The serum iron concentration and Total Iron Binding Capacity (TIBC) status of 24 patients of oral squamous cell carcinoma (OSCC) were compared with the findings of 13 healthy subjects. OSCC was found to have association with low serum iron level. More patients were found to be with significantly lower iron content in serum (p<0.05). But no association between serum TIBC and increased risk of ca...

Serum iron may be directly measured without protein precipitation, by incubation in an acetate buffer in the presence of a reducing agent and sulfonated bathophenanthroline. Mercaptoacetic and ascorbic acids have proved to be suitable reductants. Values obtained for serum iron by this procedure show excellent correlation with a trichloroacetic acid precipitation technic. Bilirubin interference ...

INTRODUCTION Oral submucous fibrosis (OSMF) is also considered as an Asian version of sideropenic dysphagia, wherein chronic iron deficiency leads to mucosal susceptibility to irritants, such as chillies and areca nut products. Hemoglobin (Hb) levels, in particular, serum iron levels, are considered as biochemical indicators for nutritional assessment. Serum iron content can thus be a predictor...

Background. Chronic Helicobacter pylori infection and iron-deficiency anemia (IDA) are common in adults. Although the most common causes of IDA usually arise from the gastrointestinal tract, the association between chronic Helicobacter pylori infection and anemia remains unclear. Aim. To evaluate the association of chronic Helicobacter pylori infection and IDA. Materials and Methods. We enrolle...

In a previous study of secondary lead smelter workers (males), a significant prevalence of low hemoglobin levels (less than 14 g/100 ml) was found; a statistically significant negative correlation between hemoglobin and zinc protoporphyrin was also detected. In the present study serum iron (Fe) levels and total iron binding capacity (TIBC) were included in the investigation of 111 secondary lea...