OBJECTIVE The epidemiology and antibiotic susceptibility of Shigella species changes over time. Updated susceptibility knowledge is necessary for appropriate empirical antibiotic treatment. Thus, this research aimed to study these changes in 2 time periods with an interval of 10 years. METHODS Two hundreds and three Shigella strains, isolated from stool samples of diarrheic patients at the Ce...

tant Shigella spp. Patients should avoid sex during symptomatic infections and wait for 2 negative stool cultures. Montreal public health officials investigated and counselled this patient as they do for every patient with Shigella infections. In Quebec, physicians and microbiology laboratories are notified of Shigella clusters and multidrug-resistant Shigella infections. To our knowledge, no o...

Septins, cytoskeletal proteins with well-characterised roles in cytokinesis, form cage-like structures around cytosolic Shigella flexneri and promote their targeting to autophagosomes. However, the processes underlying septin cage assembly, and whether they influence S. flexneri proliferation, remain to be established. Using single-cell analysis, we show that the septin cages inhibit S. flexner...

OBJECTIVES The genus Shigella comprises the most infectious and diarrheagenic bacteria causing severe diseases, mostly in children under five years of age. This study aimed to detect nine virulence genes (ipaBCD, VirA, sen, set1A, set1B, ial, ipaH, stx, and sat) in Shigella species (spp.) using multiplex polymerase chain reaction (MPCR) and to determine the relation of Shigella spp. from pediat...

background and objectives: strains of shigella spp. can cause shigellosis, or bacillary dysentery.that is a public health problem worldwide. the aim of this study was to describe the population structure and genetic relatedness of multidrug resistant s. sonnei and s. flexneri isolated during a one year period from children with diarrhea in tehran, iran. materials and methods: a total of 70 shig...

Background: Shigella flexneri is a pathogen responsible for shigellosis around the world, especially in developing countries. Many immunogenic antigens have been introduced as candidate vaccines against Shigella, including N-terminal region of IpaD antigen (NIpaD). Objective: To evaluate the efficiency of O-metylated free trimethyl chitosan na...

Three hundred and thirty-three Shigella isolates obtained in 1986 to 1995 were tested for their susceptibilities to 19 antimicrobial agents. Nalidixic acid resistance had emerged in 59.6% of Shigella flexneri isolates during 1994 to 1995, with all tested resistant isolates having the mutation in gyrA encoding the Ser-83 alteration. Multiresistance (resistance to four or more agents) was more co...

INSERM U 458, HBpitaI Robert Debre, Paris, France The differentiation between Shigella subspecies, and the phylogenetic position of Shigella clones within Escherichia coli clones was determined by analysis of restriction fragment length polymorphisms of rDNA (ribotyping). Seventy-f ive Shigella strains belonging to the four subspecies and 13 enteroinvasive Em coli (EIEC) strains were compared w...

A temperate phage, designated Sf6, has been isolated from Shigella flexneri 3a. Characterization of Sf6 revealed that it possesses the capacity for converting the S. flexneri 3,4 group antigen complex to group factor 6. Serological studies and chemical analysis of lipopolysaccharide from converted strains suggest that group factor 6 is a reflection of an acetylation of the preexisting 3,4 antig...