The inhibition of the model enzyme, haloalkane dehalogenase from Sphingomonas paucimobilis, was investigated by a combination of electrophoretically mediated microanalysis with a partial filling technique, followed by indirect or direct detection. In this setup, part of the capillary is filled with a buffer suitable for the enzymatic reaction (20 mM glycine buffer, pH 8.6) whereas the rest of t...

We demonstrate that the gel electrophoretic mobility-shift assay (EMSA) can be used for site-selective and quantitative monitoring of nicks in linear double-stranded DNA (dsDNA) thus allowing to expediently follow the nicking activity of enzymes or other agents targeted to a designated dsDNA site. At elevated temperature and/or in the presence of urea, DNA fragments carrying a single nick produ...

1Department of Chemical Engineering, Colorado State University, Fort Collins, CO, USA 2Departments of Chemical Engineering and Molecular & Cell Biology, University of Connecticut, Storrs, CT, USA 3Department of Environmental & Radiological Health Sciences and Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, USA Proteomic changes in Escherichia col...

For our analysis of the data from the First Annual Proteomics Data Mining Conference, we attempted to discriminate between 24 disease spectra (group A) and 17 normal spectra (group B). First, we processed the raw spectra by (i) correcting for additive sinusoidal noise (periodic on the time scale) affecting most spectra, (ii) correcting for the overall baseline level, (iii) normalizing, (iv) rec...

Backcrossing, coupled with marker or gene assisted selection, can be used to introgress a specific gene or chromosomal region from one population into another. The objective of this study was to assess the genomic structure of cattle produced by backcrossing for loci that are unlinked to a locus that was being introgressed. Genotypes of the two parental populations, their F1 progeny, and two su...

We report the athletic, physiological and mitochondrial-related genomic data of an Israeli endurance runner. He is holding the Israeli record in 10000, 5000, 1500 and 800m run, along with being one of the best Israeli 400m runners. We tested the ACTN3 R577X, and six polymorphisms in the PPARGC1A-NRF-TFAM pathway genes. The case athlete was heterozygous for the ACTN3 R577X variation and had five...

Pairing of northern hemisphere dabbling ducks normally occurs in wintering sites. Mate choice occurs when some individuals congregate in courtship parties, in which females mainly choose mates according to male behavioural dominance. The sex ratio of some species is more malebiased at northern than at southern wintering sites, and the age ratio is adult-biased in northern areas. A possible mech...

24 Haemophilus parasuis is the causative agent of Glässer’s disease and a commensal 25 coloniser of the porcine upper respiratory tract. Multiple complex factors, including the early 26 weaning of piglets and the management of high health status farms, make it a re-emerging 27 agent, responsible for a recent increase in the prevalence and severity of disease in pigs in 28 China. However, little...

________________________________________________________________________________ Abstract Megasphaera elsdenii (M.e.) NCIMB 41125 is a robust lactate utilizing strain of M.e. that is effective in minimizing the risk of ruminal acidosis in feedlot cattle. When dosed orally, cattle adapt smoothly to increasing concentrates in the diet, the incidence of digestive disturbances, morbidity and mortal...

Received: Revised: Accepted: 2013–09–31 2013–12–28 2013–12–29 A total of six horses were presented with severe generalized lesions including wheal with dermal edema, erythema, papules, pustules and angioedema. In one horse food hypersensitivity was found, while rest were diagnosed for idiopathic urticaria. Haridrakhand Brihat, which is a polyherbal combination containing haridra, nishoth, haree...