Prepulse Inhibition (PPI), the objective measure of sensorimotor gating disturbance has being widely used in animal models of schizophrenia. Dopaminergic direct and indirect agonists impair PPI. However, the profile of dopaminergic receptors involved in PPI impairment by dopamine agonists is not clear. By injecting shRNA expressing plasmids against dopamine D2 receptor genes (DRD2) in the nucle...

Background: HLA-G is a nonclassical HLA class I molecule which, when elevated in tumor cells, is one of the main factors involved in tumor evasion of immune responses including NK and T cells. Objective: To evaluate the effect of HLA-G downregulation on NK cell cytotoxicity in tumor cell lines. Methods: The expression level of HLA-G was measured by real-time PCR and flowcytometry after transfec...

Background: Cervical cancer is the second most common female cancer worldwide. Inhibitors of apoptosis proteins (IAPs) block apoptosis; therefore, therapeutic strategies targeting IAPs have attracted the interest of researchers in recent years. Apollon, a member of IAPs, inhibits apoptosis and cell death. RNA interference is a pathway in which small interfering RNA (siRNA) or shRNA (short hairp...

Klotho is a recently identified antiaging gene. Brain endothelin-1 (ET1) is important in the regulation of blood pressure (BP). We hypothesized that silence of brain klotho potentiates cold-induced elevation of BP via the endothelin pathway. To silence brain klotho, we constructed adeno-associated virus (AAV) carrying rat klotho small interference hairpin RNA (KL-shRNA). AAV carrying ET1-shRNA ...

STAT3 is expressed in neural stem cells (NSCs), where a number of studies have previously shown that involved regulating NSC differentiation. However, the possible molecular mechanism and role spinal cord injury (SCI) remain unclear. In present study, potential effect NSCs was first investigated by using short hairpin RNA (shRNA)‑mediated knockdown rat in vitro. Immunofluorescence β3‑t...

objective: e1a oncoprotein of adenovirus type 5 is a regulatory factor which controls transcription of other adenovirus genes. this protein promotes both viral genom replication and host cell transformation by altering the function of certain important cellular proteins such as p21 and rb. the aim of the present study was to constantly reduce the expression of e1a gene in hek 293 cell line by r...

The aim of the present study was to screen the enzymes that are associated with the radiosensitivity of SW579 thyroid cancer cells, and investigate whether radiation, combined with specific RNA interference on the screened enzymes, enhances radiosensitivity of SW579 thyroid cancer cells. Quantitative polymerase chain reaction (qPCR) was used to analyze epigenetic enzyme expression changes befor...

RNA interference (RNAi) by means of short hairpin RNA (shRNA) has developed into a powerful tool for loss-of-function analysis in mammalian cells. The principal problem in RNAi experiments is off-target effects, and the most vigorous demonstration of the specificity of shRNA is the rescue of the RNAi effects with a shRNA-resistant target gene. This presents its own problems, including the unpre...

Abstract Objectives: The mechanosensitive Ca 2+ion channels, Piezo1, converts the mechanical stimulus into cell signals in a variety of immune cells. However, pathophysiological property Piezo1 oral polymicrobial infectious disease periodontitis is largely unknown. This study investigated role inflammatory and phagocytic reactions by RAW264.7 macrophages response to exposures periodontal pathog...