Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals. The sequential phases of silver staining are protein fixation, then sensitization, then silver impregnation, and finally image development. Several variants of ...

The purpose of this study was to compare the immunoreactivity in canine renal tissues stained with antisera specific for 3 leptospiral antigens and those processed with traditional staining methods. In addition, immunoglobulin staining was done on tissues with immunoreactivity to leptospiral antigens. Formalin-fixed renal sections from 12 dogs with chronic interstitial nephritis suspected or pr...

OBJECTIVE This study was undertaken to determine whether the expressions of 3 proliferation markers (Ki-67, proliferating cell nuclear antigen, and silver-staining nucleolar organizer regions) and of p53 tumor protein could differentiate spontaneous abortions from gestational trophoblastic diseases and also discriminate among gestational trophoblastic disease subgroups. STUDY DESIGN Twenty-tw...

Silver staining was introduced more than a decade ago as a sensitive procedure to detect trace amounts of proteins in polyacrylamide gels (1). Since then, silver staining has been perfected and extended to the study of other biological molecules that have been separated by electrophoresis in a variety of supports (2,3). Silver staining follows generally one of two methods. One uses diamine or a...

Polyacrylamide gel electrophoresis (PAGE), which is widely used to assess protein purity and molecular weight [1], is best combined with a highly sensitive, reproducible, inexpensive, rapid, and easy to perform protein staining/detection method. Coomassie brilliant blue (CBB)1 staining is a popular method, known for its simplicity, economy, and compatibility with downstream analysis but relativ...

Silver nitrate staining of decalcified bone sections is known to reveal osteocyte canaliculi and cement lines. Nucleolar Organising Regions (NOR) are part of the nucleolus, containing argyrophilic proteins (nucleoclin/C23, nucleophosmin/B23) that can be identified by silver staining at low pH. The aim of this study was to clarify the mechanism explaining why AgNOR staining also reveals osteocyt...

(Running title): overview of silver staining methods for proteins ABSTRACT On the basis of the physico-chemical principles underlying silver staining of proteins, which are recalled in this paper, several methods of silver staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested. The most valuable protocols are presented in this report, including...