10.1016/S1674-6384(15)60065-X Objective To study the pharmacokinetic properties of Fufang Baijiezi Gel (FBG) after different acupoints administration. Methods Sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin, which were four substances of FBG, were determined by a sensitive liquid chromatography tandem mass spectrometry method (LC-MS) both in plasma and dermal microdialysates...

In 1825 Henry an(l Garot ( 5) isolated an alkaloidlal amine from black mustard seedls. This new natural product was namiied sinapine, an(l in 1897 Gadamer (4) propased its structure (fig 1) to be that of the choline ester of sinapic aci(l. Later studies (10, 11) showed that siniapine is wi(lelv (listributedl among members of the family Cruciferae. This compoun(l appeared to be of bioclhelmlical...

CYP84 is a recently identified family of cytochrome P450-dependent mono-oxygenases defined by a putative ferulate-5-hydroxylase (F5H) from Arabidopsis. Until recently F5H has been thought to catalyze the hydroxylation of ferulate to 5-OH ferulate en route to sinapic acid. Sinapine, a sinapate-derived ester in the seeds, is antinutritional and a target for elimination in canola meal. We have iso...

Four different parts, hypocotyl and radicle (HR), inner cotyledon (IC), outer cotyledon (OC), seed coat and endosperm (SE), were sampled from mature rapeseed (Brassica napus L.) by laser microdissection. Subsequently, major secondary metabolites, glucosinolates and sinapine, as well as three minor ones, a cyclic spermidine conjugate and two flavonoids, representing different compound categories...

Hydroxycinnamoyltransferases which catalyze the formation of O-sinapoylcholine (sinapine) using l-0-sinapoyl-ß-D-glucose as acyl donor have been isolated from seeds of radish (Raphanus sativus L. var. sativus) and mustard (Sinapis alba L.) and purified 420and 293-fold, respectively. The enzymes (“sinapine synthase”) had apparent molecular weights of about 60,000 daltons and showed highest activ...

Protein preparations from Arabidopsis thaliana and Brassica rapa catalyzed the formation o f O-sinapoyl-L-malate using 1-O-sinapoyl-ß-glucose as acyl donor. The enzyme involved, 1-O-sinapoyl-ß-glucose :L-malate O-sinapoyltransferase (SMT; EC 2 .3 .1 .-) , catalyzes in the cotyledons o f the seedlings the key step in the conversion o f the seed constituent sinapine (O-sinapoylcholine) to sinapoy...

Raphanus sativus, Brassicaceae, Sinapic Acid Esters, Phenylpropanoids, High Performance Liquid Chromatography Five sinapic acid esters were isolated from cotyledons of Raphanus sativus and have been iden­ tified with the aid of electron impact (El)-, field desorption (FD)-mass spectrometry and NMR spectroscopy: 6,3'-disinapoylsucrose, sinapoylcholine (sinapine), 6 -sinapoylglucoraphenine, 1 -si...