نتایج جستجو برای: six primer pairs pb
تعداد نتایج: 475275 فیلتر نتایج به سال:
Six New World Camelidae microsatellite primer pairs were used to investigate the genetic polymorphism in Jaisalmeri camel. Polymerase chain reactions were carried out for 30 unrelated camels of Jaisalmeri breed. The amplification products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. All the 6 microsatellite primer pairs were found polymorphic in Jaisalmeri camel. ...
Eleven new microsatellite markers were isolated from taro, Colocasia esculenta (L.) Schott, a root crop widely distributed all over the world. Forty-eight primer pairs were designed from a microsatellite-enriched genomic library, of which 11 primer pairs have polymorphisms in 30 individuals tested from a population in China, which revealed two to six alleles per locus with the observed and expe...
Ten primer pairs were screened to develop single nucleotide polymorphism (SNP) TaqMan assays that will distinguish California golden trout and some rainbow trouts (Oncorhynchus mykiss sspp., O. m. aguabonita) from the Paiute and Lahontan cutthroat trouts (Oncorhynchus clarkii seleniris, O. c. henshawi). From these 10 primer pairs, one mitochondrial and five nuclear fixed SNP differences were di...
almonds are self incompatible, the incompatibility being controlled by a multi allelic (s-alleles) single gene. the incompatibility alleles were studied in 96 samples of wild almonds and related prunus species from 10 taxonomic groups. polymerase chain reactions were carried out with six primer sets including: three degenerate primer pairs (paconsi-f(fam)/em-pc1consrd, paconsi-f(fam)/em-pc3cons...
Chloroplast microsatellites (cpSSRs) provide a powerful tool to study the genetic variation and evolution of plants. We have investigated the usefulness of 39 primer pairs tagging cpSSR loci on a set of eight different genera of Leguminosae (Papilionoideae subfamily) and five species belonging to the genus Phaseolus. Thirty-six 'universal' primer pairs were retrieved from the literature, one wa...
We investigated the utility of the Gossypium arboreum EST sequences in the GenBank database for developing PCR-based markers targeting known-function genes in cultivated tetraploid cottons, G. hirsutum and G. barbadense. Four hundred sixty-five randomly selected ESTs from this library were subjected to BLASTn search against all GenBank databases, of which putative function was assigned to 93 ES...
A total of 2414 new di-, tri- and tetra-nucleotide non-redundant SSR primer pairs, representing 2240 unique marker loci, have been developed and experimentally validated for rice (Oryza sativa L.). Duplicate primer pairs are reported for 7% (174) of the loci. The majority (92%) of primer pairs were developed in regions flanking perfect repeats > or = 24 bp in length. Using electronic PCR (e-PCR...
Rice (Oryza sativa L.) is one of the most important crops in the world. It is essential to develop an efficient method to build the fingerprinting database of commercial hybrid rice cultivars and their parental lines in seed market for rapid and unambiguous cultivar identification and seed purity analysis as new similar cultivars increasing yearly. The objective of this investigation were to fi...
The genus Swertia is well known for its medicinal properties, as described in the Indian pharmacopoeia. Different members of this genus, although somewhat similar in morphology, differ widely in their pharmacological and therapeutic properties. The most important species of this genus, with maximal therapeutic properties, is S. chirayita, which is often adulterated with other less-potent Swerti...
A database of 30,137 EST sequences from Mycosphaerella graminicola, the septoria tritici blotch fungus of wheat, was scanned with a custom software pipeline for di- and trinucleotide units repeated tandemly six or more times. The bioinformatics analysis identified 109 putative SSR loci, and for 99 of them, flanking primers were developed successfully and tested for amplification and polymorphis...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید