the anionic surfactant sodium dodecyl sulfate (sds) was degraded by novel strain ofpseudomonas aeruginosa kgs under accession no. jq328193, which was isolated from carwash wastewater. the purpose of this research was to study different optimization conditionsrequired for enhancing the biodegradation of sodium dodecyl sulfate p. aeruginosa kgs.influence of different physicochemical factors such ...

Epoxide hydrase was solubilized from liver microsomes of phenobarbital-treated rats by treatment with cholate and purified to apparent homogeneity by ammonium sulfate fractionation and column chromatography in the presence of the nonionic detergent Emulgen 911 on DEAE-cellulose and hydroxylapatite. The purified enzyme preparation had a single major band with a molecular weight of 53,000 to 54,0...

A vitamin Blz-binding protein has been isolated from human granulocytes derived from patients with chronic granulocytic leukemia. Utilizing affinity chromatography as the sole purification technique, the protein was purified 9,860-fold with a yield of over 90% and was homogeneous based on polyacrylamide disc gel electrophoresis, sedimentation equilibrium ultracentrifugation, and sodium dodecyl ...

A vitamin Blz-binding protein has been isolated from human granulocytes derived from patients with chronic granulocytic leukemia. Utilizing affinity chromatography as the sole purification technique, the protein was purified 9,860-fold with a yield of over 90% and was homogeneous based on polyacrylamide disc gel electrophoresis, sedimentation equilibrium ultracentrifugation, and sodium dodecyl ...

The run time for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) can be reduced to as little as 5 min by running vertical slab gels at 70°C. High-temperature SDS-PAGE increases the migration rate of protein bands several fold and is as effective as cooling in preventing uneven migration rates across the width of the gel. Additional benefits of hightemperature SDS-PAGE inclu...

A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum dir...

This study investigated calcium sulfate and chitosan on the textural modification microstructure of tofu made from lentils. The addition varying amounts (0–12 mM) (0–1.0%) into lentil milk could affect gel properties lentil-based tofu. properties, including hardness cohesiveness, significantly increased with 12 mM sulfate, exhibiting a slightly discontinuous network structure regular pore netwo...

Ornithine decarboxylase was purified to homogeneity, as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and polyacrylamide gel electrofocusing, about 710,000-fold with a 35% yield from the liver cytosol of thioacetamide-treated rats. The final specific activity was approximately 24,400 nmol/ min/mg of protein. The apparent molecular weight of the enzyme determined by gel fil...

Fructan-hydrolyzing enzyme from Streptococcus salivarius KTA-19 isolated from human dental plaque was investigated. The enzyme was purified by ammonium sulfate precipitation, acetone fractionation, and column chromatography on Bio-Gel and DEAE-cellulose. The purified enzyme showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing. Its molecular ...