A number of polyacrylamide gel systems and solubilization procedures were studied to define the number and nature of "major" polypeptide bands in the outer membrane of Pseudomonas aeruginosa. It was shown that five of the eight major outer membrane proteins were "heat modifiable" in that their mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was determined by the solubiliza...

Rabbit testis contains a single molecular form of proacrosin which could be extracted at acid pH and purified 145-fold by Sephadex G-100, SE-Sephadex, and concanavalin A-Sepharose affinity column chromatography. The purified proacrosin was judged to be homogeneous on the basis of chromatographic analyses, disc and sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis, and immunodiffusi...

The run time for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) can be reduced to as little as 5 min by running vertical slab gels at 70°C. High-temperature SDS-PAGE increases the migration rate of protein bands several fold and is as effective as cooling in preventing uneven migration rates across the width of the gel. Additional benefits of hightemperature SDS-PAGE inclu...

Fructan-hydrolyzing enzyme from Streptococcus salivarius KTA-19 isolated from human dental plaque was investigated. The enzyme was purified by ammonium sulfate precipitation, acetone fractionation, and column chromatography on Bio-Gel and DEAE-cellulose. The purified enzyme showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing. Its molecular ...

Exo-alpha-N-acetylgalactosaminidase has been purified 8000-fold from Clostridium perfringens by gel filtration, ion exchange chromatography, isoelectric precipitation, and negative adsorption on human O type erythrocytes. The resulting enzyme is active at physiological pH and temperature. Phenyl glycosides, oligosaccharides, mucins, glycolipids, and cell membranes are substrates for this enzyme...

This study investigated calcium sulfate and chitosan on the textural modification microstructure of tofu made from lentils. The addition varying amounts (0–12 mM) (0–1.0%) into lentil milk could affect gel properties lentil-based tofu. properties, including hardness cohesiveness, significantly increased with 12 mM sulfate, exhibiting a slightly discontinuous network structure regular pore netwo...

Membrane-associated lipoxygenase from green tomato (Lycopersicon esculentum L. cv Caruso) fruit has been purified 49-fold to a specific activity of 8.3 mumol.min(-1).mg(-1) of protein by solubilization of microsomal membranes with Triton X-100, followed by anion- exchange and size-exclusion chromatography. The apparent molecular mass of the enzyme was estimated to be 97 and 102 kD by sodium dod...

A new apoprotein has been isolated from delipidated human very low density lipoproteins by Sepharose 6B chromatography in 6 Y guanidine hydrochloride. This water-insoluble protein was noted to be unique to the plasma very low density and chylomicron fractions. This protein was shown to be homogeneous by urea-DEAE-cellulose chromatography, rechromatography in a guanidine-Sepharose system, sodium...

Glass wool column chromatography was used for separation of the two glycoproteins of western equine encephalitis virus. Cross-contamination of each protein separated was confirmed to be negligible by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.