When aggregated micelles of phospholipids are hydrolysed by phospholipase A2 (PLA2) in an aqueous dispersion, scattering from the solution is decreased. Hydrolysis of dimyristoyl phosphatidylcholine by PLA2 from Russell’s viper venom has been investigated using a spectrofluorimeter at 650 nm. The rate of decrease in scattering was linearly dependent with venom concentration, while the initial l...

Abstract The seed shell of soybeans performs a protective function and participates in the vital processes seed. Soybean seeds are characterized by unique chemical composition. priority direction development technologies for production foodstuffs from is control quality indicators their shells. use spectrofluorimeter an IR-Fourier spectrometer allows you to study coating material soybean quickl...

The paper studies the processes of transformation molecular structure humic (HA) and fulvic acids (FA) leached chernozem under influence fertilizers meliorant. research was carried out conditions many years stationary experiment Department Agrochemistry, Soil Science Agroecology FSBEI HE Voronezh SAU. Samples low-humus thin heavy-loamy were analyzed. isolated purified preparations HA FA studied...

The spectral evaluation systems for controlling the feed mixtures homogeneity were analyzed. ( Research purpose ) To study optical luminescent properties of main components in ultraviolet and visible range develop a method their photoluminescent quality control. Materials methods Two groups mixture studied: granular compound corn silage. characteristics measured by Fluorat-02-Panorama spectrofl...

The present study was designed to explore the mechanisms involved in the anti-ischemic action of lumbrokinase (LK) in brain. The enzyme immunoassay, spectrofluorimeter and flow cytometry were used to detect the level of adenosine 3',5'-cyclic monophosphate (cAMP) and guanosine 3',5'-cyclic monophosphate (cGMP), the Ca(2+) mobilization, and human platelet surface antigen expression in order to e...

A 15-day survey of autofluorescence has been conducted upon infection by downy mildew [Plasmopara viticola (Berk. & M.A. Curtis) Berl. & de Toni] of leaves of a susceptible grapevine genotype. Different autofluorescence signals were followed from the cellular to the whole-leaf level by using four types of devices for fluorosensing: a macroscope, a spectrofluorimeter, a portable field optical se...

Both vitamin E and thymoquinone have been used in the treatment of many neurodegenerative disorders. This study aims to investigate the effect of vitamin E or thymoquinone on the alterations in monoamine neurotransmitters induced by nicotine treatment and withdrawal in adult male albino rats. Animals were divided into control group (0.9% NaCl saline solution), nicotine-treated group (1mg/kg B.W...

In the present work we investigated the possible role of the native fluorescence of blood plasma in the management of colorectal cancer (CRC) and its feasibility as a new tumor marker. Sample of blood was collected from 248 asymptomatic blood donors and from 246 CRC patients. The native fluorescence of blood plasma was measured using a conventional spectrofluorimeter. The intensity of fluoresce...

The application of dyes, that fluoresce in the near infrared (NIR, 700-800 nm) region, for recognition samples using a fingerprinting method with addition fluorophores to (“fluorescent eye”) is proposed. technique has been successfully applied classification various nature. In current work, this strategy tested on example discrimination 17 apple juice from different manufacturers, purchased at ...

A spectrometric technique is presented that combines most of the important criteria necessary for efficient detection and identification of microorganisms. These criteria include simplicity of experimental design, various degrees of sensitivity and selectivity, convenience, and total reaction times of less than 15 min. The study takes advantage of the inherent extracellular enzymes present in l...