sperm dna is known to contribute one half of the genomic material to the offspring. the integrity of sperm dna is important in fertilization, embryonic and fetal development, and postnatal child well being. the nature has created multiple barriers that allow only the fittest sperm to reach and fertilize an oocyte. however, assisted reproductive techniques (art), like ivf and icsi, may allow spe...

Evidence supports the involvement of nitric oxide (NO) in a Varity of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. However, Low concentration of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. On the other hand, it is established t...

this study was conducted to determine chromatin integrity and dna damage by dna electrophoresis and comet assays of buffalo fresh and frozen semen. semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and dna damage. a significant variation was found in semen parameters after thawing. highly sig...

Introduction: The novel development of the (HBA) sperm- hyaluronan binding assay has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. The purpose of this study was to evaluate the correlation between HBA score and histone deficiency, protamine content and DNA fragmentation. Methods: During April- March 2012 patients who were enrolled at ...

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

DNA fragmentation in human sperm has been related to endogenous and exogenous factors. Exogenous factors can also affect leukocyte DNA integrity. This study evaluated the relation between sperm DNA damage and leukocyte DNA integrity, as a predictor of exogenous factors. DNA damage in the sperm and leukocytes of 41 individuals undergoing ICSI were measured by Comet assay. In addition, sperm chro...

objective: the aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or bioxcell®) on cryopreservation of buffalo semen. materials and methods: in this experimental study, split pooled ejaculates (n=4), possessing more than 70% visual sperm motility were divided in two aliquots and diluted in bioxcell ® and tris-citric...

Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and DNA integrity of rat spermatozoa during semen freeze-tha...

objective: the aim of this study was to examine the efficiency of ha-binding test and sperm gradient processing method for the selection of sperm with normal protamine, intact dna and normal morphology. materials and methods: the study was conducted on 29 patients undergoing icsi. semen analysis was carried out according to who criteria. semen samples were divided into three equal portions. one...