Semen samples from 9 sexually mature dogs of different breeds, aged from 1 to 5 years were used. Quality of sperm was evaluated using the following parameters: progressive motility (subjective), then structural and functional integrity of spermal membrane (supravital colouring (eosine/nigrosine) and HOS test). The majority of HOS reactive or swollen spermatozoa was placed in a hypoosmolar solut...

The process of fertilization can’t be attributed only to the number of live, motile and normal sperm deposited within the female but especially to its functionality [1]. The hypoosmotic test or hypoosmotic test (HOST) is a functional test, by which the live sperm are subjected to an incubation in a hypoosmotic solution, the sperm with the functional membrane will allow the entry of water by osm...

The process of fertilization can’t be attributed only to the number of live, motile and normal sperm deposited within the female but especially to its functionality [1]. The hypoosmotic test or hypoosmotic test (HOST) is a functional test, by which the live sperm are subjected to an incubation in a hypoosmotic solution, the sperm with the functional membrane will allow the entry of water by osm...

Background: During ICSI, motile sperm with normal morphology are selected and injected into oocyte. But integrity of chromatin is not observable. Therefore, researchers are trying for to present novel method of sperm selection with high percentage of normal chromatin. HOST test is a useful assay of functional integrity of the human sperm membrane. We decide to use HOST test after DGC for infert...

Accurate prognosis of male fertility based on semen measurements is still not straightforward. This study was designed to identify the best predictors of fertility and to develop a multiple regression model predicting fertility using selected parameters of semen analysis. The predictive value of standard semen parameters and selected functional tests were studied in 113 fertile men and in 109 s...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. Human assisted reproduction results have improved dramatically in the last 20 years, with lower complications for the patient as well as for the newborns. This requires a precise clinical diagnosis that will allow us to individualize the best-personalized treatment for each patient or couple. But still today, the basi...

AIM To assess the contraceptive efficacy of the benzene chromatographic fraction of the chloroform extract of the seeds of Carica papaya in langur monkeys. METHODS The test substance was given p.o. to five monkeys at 50 mg/kg body weight/day for 360 days. Control animals (n=3) received olive oil as vehicle. Sperm parameters as per World Health Organization standards, sperm functional tests, m...

sperm is produced by the testis and mature in the epididymis. for having a successful conception, the fertilizing sperm should have functional competent membranes, intact acrosome, functional mitochondria and an intact haploid genome. the effects of genetic and environmental factors result in sperm vulnerability to damage in the process of spermatogenesis and maturation. in recent years, the fe...

Aim of the study: to evaluate the functional parameters of bovine spermatozoa before and after separation by single layer centrifugation using Bovipure gradient. Material and Methods: Frozen semen from 8 bulls was used in this study. Thawed pellets were subjected to the separation process through gradient media according to the manufacturer’s instructions. Qualitative assessment of semen was pe...