the complex process of spermatogenesis is regulated by various factors. studies on spermatogonial stem cells (sccs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. to investigate and biomanipulation of these cells, proliferation and viability rate of cells should be ...

introduction: nowadays, spermatogonial stem cells (sscs) cultivation has been used by many researchers as an effective tool for infertility treatments. oxidative conditions can be effective on cell proliferation and differentiation of these cells. so, the aim of this study was to establish oxidative stress model for antioxidant activity of some drugs investigation during sscs in vitro culture. ...

CONTEXT Young boys treated with high-dose chemotherapy are often confronted with infertility once they reach adulthood. Cryopreserving testicular tissue before chemotherapy and autotransplantation of spermatogonial stem cells at a later stage could theoretically allow for restoration of fertility. OBJECTIVE To establish in vitro propagation of human spermatogonial stem cells from small testic...

background: spermatogonial stem cells are subpopulation of spermatogonial cells in testis tissue that support beginning and maintenance of spermatogenesis. ubiquitin carboxy-terminal hydrolase l1 (uchl1) could be a specific marker for identification of spermatogonial stem cells including spermatogonial sperm cells (sscs) in testis tissue and during the culture; therefore we undertook this study...

spermatogonia are the male germ line stem cells whose life long expansion is needed for permanent production of spermatozoa. the present study was designed to examine the effect of hcg treatment on germ cell proliferation following stem cell transplantation in mice. spermatogonial stem cells were isolated from neonatal mice testes and characterized by alkaline phosphatase, immunoreactivity and ...

BACKGROUND To isolate and characterization of human spermatogonial stem cells from stem spermatogonium. METHODS The disassociation of spermatogonial stem cells (SSCs) were performed using enzymatic digestion of type I collagenase and trypsin. The SSCs were isolated by using Percoll density gradient centrifugation, followed by differential surface-attachment method. Octamer-4(OCT4)-positive SS...

background: the complex process of spermatogenesis is regulated by various factors. studies on spermatogonial stem cells have provided a very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules consist of spermatogonial stem cells. to investigate and biomanipulate these cells, first the proliferation and viability rate of cells s...

background herbal extracts have recently received the greatest attention in the path of finding naturally occurring chemicals with antibacterial and therapeutic value; however, each type of herbal remedy may have its own side effects. objectives the aim of the current experiment was to study the antibacterial effect of myrtle, parsley, mint, henna and chamomile extracts on escherichia coli and ...

Stem cells give rise to differentiated cell types but also preserve their undifferentiated state through cell self-renewal. With the use of transgenic mice, we found that the RNA-binding protein NANOS2 is essential for maintaining spermatogonial stem cells. Lineage-tracing analyses revealed that undifferentiated spermatogonia expressing Nanos2 self-renew and generate the entire spermatogenic ce...

background: spermatogonial stem cells (sscs), a subset of undifferentiated type a spermatogonia, are the foundation of complex process of spermatogenesis and could be propagated in vitro culture conditions for long time for germ cell transplantation and fertility preservation. objective: the aim of this study was in vitro propagation of human spermatogonial stem cells (sscs) and improvement of ...