Abstract Spheroids have become essential building blocks for biofabrication of functional tissues. Spheroid formats allow high cell-densities to be efficiently engineered into tissue structures closely resembling the native In this work, we explore assembly capacity cartilaginous spheroids ( d ? 150 µ m) in context endochondral bone formation. The fusion at various degrees differentiation was i...

Gaussian beam scattering by a spheroidal particle is studied in detail. A theoretical procedure is given to expand an incident Gaussian beam in terms of spheroidal vector wave functions within the generalized Lorenz-Mie theory framework. Exact analytic solutions are obtained for an arbitrarily oriented spheroid with nonconfocal dielectric coating. Normalized differential scattering cross sectio...

We analyze 166 spheroid subdwarfs found in 53 Ðelds observed with the Wide Field (6.5\ M V \ 14.5) Planetary Camera on the Hubble Space T elescope. The Ðelds cover 221 arcmin2 over a wide range of directions. The spheroid luminosity function (LF) is inconsistent at about the 3 p level with the local spheroid LF of Dahn et al. even when the normalization of the latter is corrected to take accoun...

We analyze 166 spheroid subdwarfs (6.5 < MV < 14.5) found in 53 fields observed with the Wide Field Camera on the Hubble Space Telescope. The fields cover 221 arcmin2 over a wide range of directions. The spheroid luminosity function (LF) is inconsistent at about the 3 σ level with the local spheroid LF of Dahn et al. even when the normalization of the latter is corrected to take account of the ...

OBJECTIVE To assess relative biological effectiveness (RBE) of (131)I radiation relative to (60)Co gamma rays in glioblastoma spheroid cells. MATERIALS AND METHODS : In this experimental study, glioblastoma spheroid cells were exposed to (131)I radiation and (60)Co gamma rays. Radiation induced DNA damage was evaluated by alkaline comet assay. Samples of spheroid cells were treated by radiati...

The cancer stem cell (CSC) theory hypothesizes that CSCs are the cause of tumor formation, recurrence and metastasis. Key to the study of CSCs is their isolation and identification. The present study investigated whether spheroid body-forming cells in the human gastric cancer (GC) MKN-45 cell line are enriched for CSC properties, and also assessed the expression of the candidate CSC markers, oc...

Three-dimensional spheroid cultures have become increasingly popular as drug screening platforms, especially with the advent of different high throughput spheroid forming technologies. However, comparing drug efficacy across different cell types in spheroid culture can be difficult due to variations in spheroid morphologies and transport characteristics. Improving the reproducibility of compact...

the purpose of this study is to investigate the effects of quercetin as an inhibitor of heat shock proteins and hyperthermia on the induced dna damages and colony formation ability of du145 tumor spheroid culture. du145 cells were cultured as spheroids. on day11, spheroids with mean diameter 100 μm were treated with different concentration of quercetin for 24 hours and then exposed to hyperther...

We describe and share a device, methodology and image analysis algorithms, which allow up to 66 spheroids to be arranged into a gel-based array directly from a culture plate for downstream processing and analysis. Compared to processing individual samples, the technique uses 11-fold less reagents, saves time and enables automated imaging. To illustrate the power of the technology, we showcase a...

The endothelial cell spheroid assay provides a suitable in vitro model to study (lymph) angiogenesis and test pro- and anti-(lymph) angiogenic factors or drugs. Usually, the extent of cell invasion, observed through optical microscopy, is measured. The present study proposes the spatial distribution of migrated cells as a new descriptor of the (lymph) angiogenic response. The utility of this no...