The role of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) immediate-early protein IE0 in the baculoviral infection is not clear. In this study, we constructed the recombinant virus vAcDeltaie0 null for ie0 expression by targeted mutagenesis replacing exon0 with the cat gene. We found that vAcDeltaie0 replicated efficiently in Spodoptera littoralis SL2 cells, which are poorly...

20-Hydroxyecdyone (20E), an active form of ecdysteroid, is the key hormone in insect growth and development. To date five P450 enzymes involved in the last four steps of ecdysteroid biosynthesis from ketodiol to 20E namely Spook (Spo), Phantom (Phm), Disembodied (Dib), Shadow (Sad) and Shade (Shd) related to ecdysteroid biosynthesis, are identified and the character of last four enzymes is well...

The insect antifeedant and toxic activity of hydroxyperezone (1), its derivatives 2-9, along with 3-hydroxy- (10) and 6-hydroxythymoquinone (11) were studied against Spodoptera littoralis, Leptinotarsa decemlineata, and Myzus persicae. The antifeedant tests showed that L. decemlineata was the most sensitive insect, followed by M. persicae, while S. littoralis was not deterred by compounds 1-11....

The authors imparted the main concept of infochemical communication and its realization in the first micromachinebased chemoemitter. We present herein details on the microfabrication and functionality of its elements, particularly the biomicroreactor and the evaporator. The functionality of the integrated chemoemitter has been evidenced in an electrophysiological assay using antennae of male Sp...

A practical, brief, and diastereoselective synthesis of limonoid CDE fragments from a readily available starting material is described. The key step was the titanocene(III)-promoted cyclization of unsaturated epoxylactones, readily prepared from α-cyclocitral. In this way, we confirm the viability of our procedure for the synthesis of a limonoid model with different functionalization patterns. ...

Bacillus thuringiensis insecticidal crystal proteins (ICPs) are thought to induce pore formation in midgut cell membranes of susceptible insects. Cry1Ca, which is significantly active in Spodoptera littoralis, made brush border membrane vesicles permeable to KCl (osmotic swelling was monitored by the light scattering technique); the marginally active ICPs Cry1Aa, Cry1Ab, and Cry1Ac did not.

The aim of this study was to determine the insecticide potential essential oils (EOs) thyme (Thymus vulgaris; Lamiaceae) and ginger (Zingiber officinale; Zingiberaceae) control cotton leaf worm (Spodoptera littoralis; Lepidoptera: Noctuidae) under laboratory conditions. Generally, there a positive correlation between magnitude above toxic effects concentrations all EOs. Ginger oil (GEO) showed ...

A new formula for insect rearing artificial diets without using any of their conventional gelling agents was provided in the present work, for the first time. Larvae of the Egyptian cotton leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae) were successfully mass-reared, for nine consecutive generations on an agar-free artificial diet. This suggested medium is based on yellow lentils, Lens...

Biochemical and histological analysis were performed on 4 larval instar of the cotton leaf worm Spodoptera littoralis to determine the effect of abamectin (LC50) on the biomarkers acetylcholinesterase (AChE), glutathione S-transferase (GST), α-, βesterase and mixed function oxidase (MFO).Biochemical analysis showed a severe reduction in the enzymatic activities of GST and α-esterase, as compare...