microbial calcium carbonate, by bridging sand particles, can play an important role in sand dune stability. a study was carried out on the cementation of sand grains and infilling of pore spaces by caco3. two bacterial species (sporosarcina pasteurii and sporosarcina ureae), three reactant concentrations (0.5, 1 and 1.5m), and six reaction times (12, 24, 48, 96, 192 and 288 hours) were tested i...

The particular bacterium under investigation here (S. pasteurii) is unique in its ability, under the right conditions, to induce the hydrolysis of urea (ureolysis) in naturally occurring environments through secretion of an enzyme urease. This process of ureolysis, through a chain of chemical reactions, leads to the formation of calcium carbonate precipitates. This is known as Microbiologically...

Sporosarcina pasteurii is known to produce calcite or biocement in the presence of urea and Ca(2+). Herein, we report the use of novel ultramicrosensors such as pH, Ca(2+), and redox sensors, along with a scanning electrochemical microscope (SECM), to monitor a real-time, bacteria-mediated urea hydrolysis process and subsequent changes in morphology due to CaCO3 precipitation. We report that th...

Using a soil improvement biotechnology based on microbial-induced carbonate precipitation (MICP) to form horizontal water barrier in foundation pit dewatering can not only achieve good control effect, but also the cost and obtain economic environmental benefits. In this study, taking Shanghai layer ⑨ sand as research object, solution injected into by MICP grouting technology includes bacterial ...

Biocement can be achieved through the microbially induced carbonate precipitation (MICP) process. Such a method potentially utilized as an eco-friendly for civil and environmental engineering applications such soil ground improvement wind erosion control of surface soil. In this method, one key step is effective production ureolytic bacteria. previous laboratory field studies, cultivation bacte...

Calcareous sand, as a blow-fill or construction material, is widely used in island and reef projects marine environments after treatment. When microorganism-induced mineralization to strengthen calcareous salinity other conditions the environment will adversely affect microorganisms their process. For this reason, two environmental created by deionized water simulated seawater were introduced e...