We describe a rapid and simple enumeration method for sulphate-reducing bacteria (SRB) from aquatic environments using a quantitative real-time PCR (qPCR) in combination with a rapid DNA isolation method. Enumeration of SRB in the sediment and water samples was performed by quantifying the copy number of the dsrA gene coding for the a-subunit of the dissimilatory sulphite reductase using real-t...

BACKGROUND Anisochilus carnosus (L.f.) wall (Lamiaceae), an annual herb which grows at high altitude is used extensively in folk medicine for the treatment of ailments such as gastric ulcer and skin diseases. The aim of our study was to evaluate the anticancer activity of different extracts of the leaves of A.carnosus. An attempt was also made to estimate the luteolin content in different extra...

Some novel fused heterocyclic compounds of 2,5-disubstituted-benzoxazole derivatives, which were previously synthesized by our group, were investigated for their mutagenic properties on Salmonella typhimurium TA 98 and TA 100 strains, cytotoxic activity in L929 and HeLa cell lines by Sulforhodamine B (SRB) cytotoxicity test, and genotoxic potentials in the comet assay. By using Ames/Salmonella ...

BACKGROUND The plant Launaea procumbens belongs to the family Asteraceae and traditionally used in the treatment rheumatism, kidney, liver dysfunctions and eye diseases. In the present study Phytochemical analysis and fractions of methanolic extract of L. procumbens leaves were tested in vitro for their cytotoxicity. OBJECTIVES Phytochemical analysis and cytotoxic activity of methanolic extra...

BACKGROUND: Osteosarcoma (OS) is considered extremely rare type of bone tumor although it the most common malignant in children with less occurrence elderly patients. Herbal plants and phytoconstituents are recently used treatment OS to avoid side effects chemotherapeutic drugs. AIM: The aims present study investigate effect natural compound Vitamin C, green tea, their combination on cell line ...

One of the deadliest cancers among women is a breast cancer. Research has shown that two natural substances occurring in propolis, caffeic acid (CA) and caffeic acid phenethyl ester (CAPE), have significant anticancer effects. The purpose of our in vitro study was to compare cytotoxic activity and migration rate inhibition using CA and CAPE (doses of 50 and 100 µm) against triple-negative, MDA-...

Atranorin 1, Usnic acid 2, and D-Arabinitol 3 were isolated from Usnea aciculifera by extensive column chromatography. The structures elucidated based on the comprehensive spectral analysis including FT IR, GC MS, ESI 13C NMR, 1H NMR. compounds evaluated for their cytotoxic activity against LN-229 glioblastoma cancer cell line performing Sulforhodamine B assay (SRB). 2 showed strong with an IC5...

Some 2-thiouracils and fused 2-thiouracils were evaluated for their cytotoxicity study .The synthesized compounds were tested for their cytotoxicity effects towards (CaCo-2) cancer cell line using SRB assay. The results showed that compound 1b displayed promising cytotoxic activity and found to be more potent than standard, doxorubicin.

Four sorbicillinoids (1-4) and seven bisorbicillinoids (5-11), including two new compounds, 6-demethylsorbicillin (1) and 10,11-dihydrobisvertinolone (6), were isolated from a marine-derived fungus Trichoderma sp. Their cytotoxic activities against HL-60 cell line were evaluated by Sulforhodamine B (SRB) assay method and flow cytometric analysis.

Objective: This study is to investigate the antioxidant and antitumor effects of the extract fractions of the Ferula sinkiangensis K. M. Shen. Methods: Four different fractions of the Ferula sinkiangensis K. M. Shen were obtained by the extraction with petroleum ether, ethyl acetate, n-butanol, and methanol, respectively, which were used to treat the HCT116, Caco-2, HepG2, and MFC cells. Free r...