We present a sensitive colorimetric determination of 5'-nucleotidase based on the measurement of liberated phosphate by reaction with stannous chloride/molybdenum blue. No protein precipitation is required. The method is simple, sensitive, economical, and easy to use, and involves relatively stable reagents.

PURPOSE The authors have previously shown a marked increase in corneal epithelial arachidonic acid metabolism to 12-hydroxyeicosatetraenoic acid (12-HETE) and 12-hydroxyeicosatrienoic acid (12-HETrE) in a model of closed eye-contact lens wear. Their formation was predominantly cytochrome P450-dependent and significantly correlated with inflammatory score and corneal thickness. In the current st...

OBJECTIVES This study aimed to analyse the erosion-inhibiting potential of a single application of stannous chloride-containing fluoride solution on pellicle-covered enamel and dentine under constant acid flow conditions in vitro. DESIGN Bovine enamel (n=60) and dentine (n=60) samples were exposed 1h to the oral cavity of 4 healthy volunteers to allow for in situ pellicle formation. Pellicle-...

Methods for the determination of phosphorus depend almost entirely upon the reduction of phosphomolybdic acid with the formation of a complex blue pigment which is presumably a mixture of molybdenum oxides. Many variations of methodology revolve about the means by which the colored complex is obtained. The method of Fiske and Subbarow (2), in which aminonaphtholsulfonic acid (ANS) is utilized, ...

The reaction of 5-nitrobenzimidazolone with phenoxyethyl bromide in presence of potassium carbonate in dimethyl formamide obtained 6-nitro-1,3-bis(2-phenoxyethyl)-1,3-dihydro-2H-benzimidazol-2-one. It was reduced using stannous chloride to get 6-amino -1,3-bis(2-phenoxyethyl)-1, 3-dihydro-2H-benzimidazol -2-one, which was further treated with aromatic sulphonyl chloride to obtain benzimidazolon...