conclusions our data suggest that preconditioning with 5 μm h2o2 + 0.5% fbs and 10 μm h2o2 + 5% fbs improved cell proliferation and viability. however, the mechanisms related to protective properties of preconditioning and using this strategy in stem cell therapy requires more research. background mesenchymal stem cells (mscs) survival decreased following in vivo injection and its application h...

background: due to increasing clinical demand for adipose tissue, a suitable cell for reconstructive adipose tissue constructs is needed. in this study, we investigated the ability of human endometrial-derived stem cells (enscs) as a new source of mesenchymal stem cells to differentiate into adipocytes. enscs are the abundant and easy available source with no immunological response, for cell re...

in this study, our aim was to produce a generation of gmp-grade adipose tissue-derived mesenchymal stem cells for clinical applications. according to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cgmp) compliant adipose tissue-derived mesenchymal stem cells from five female donors. the isolated cells were cultured in dmem supplemented...

introduction: hyperplastic pulpitis (pulp polyp)tissues contains cells with stem cell properties similar to that of the dental pulp stem cells (dpscs). it has also been shown that cd146 enrichment can homogenize the cultures of dpscs and enhance the colony forming potentials of their cultures. this study determines whether cd146 enrichment can help purifying the stem cells from heterogeneous cu...

purpose: to describe a novel surgical technique for in vivo limbal stem cell cultivation for reconstruction of corneal epithelium in total limbal stem cell deficiency (lscd). methods: patients suffering from chronic total unilateral lscd due to a chemical injury were sequentially included. one small limbal block (2x1 mm) harvested from contralateral healthy eye sutured over the amniotic membran...

tissue and cell transplantation are regarded as a popular procedure in clinical sciences, prospecting a new horizon for several incurable diseases. along with its usefulness, many ethical concerns accompany this development. the ethical issue of organ transplant is unique to the source used which includes: living related, living unrelated, cadaveric, and xenotransplant. obtaining organs has a s...

objective(s): bone marrow-derived mesenchymal stem cells (bm-mscs) potentials make them appropriate for cell therapy including ability of differentiation and release of anti-inflammatory cytokines and growth factors secreta. for treatment of azoospermia to induce proliferation and differentiation of germ cells, mscs transplantation has been introduced. the aim of the present experimental case-c...

the provision of an adequate quantity of cells with proper function and purity is one of the main challenges of tissue engineering studies. stem cells, with their self-renewal and differentiation capacity, are considered one of the main cell sources in the field of tissue engineering. previously, the use of chemical factors seemed to be the only possible way for stem cell differentiation. howev...

objective: demyelination of cns axons occurs under pathological conditions such as multiple sclerosis and spinal cord injuries, but can be repaired by cell therapy. within the cns remyelination can be achieved by transplantation of neural stem cells (nscs). nscs are self-renewing cells that maintain the capacity to differentiate into cns-specific cell types and can differentiate into the three ...

cancer stem cells (csc) are the tumor-associated cells existed within tumors or hematological cancers which share characteristics similar to normal stem cells. the common characteristics of a normal stem cell and a csc are their differentiation capacity and self-renewal in tumors. the expression pattern of csc markers differs depending on the type and location of cancers. cd molecules are proba...