A novel milliliter-scale stirred tank bioreactor was developed for the cultivation of mycelium forming microorganisms on a 10 milliliter-scale. A newly designed one-sided paddle impeller is driven magnetically and rotates freely on an axis in an unbaffled reaction vessel made of polystyrene. A rotating lamella is formed which spreads out along the reactor wall. Thus an enhanced surface-to-volum...

We study the stochastic model for bioremediation in a bioreactor with ideal mixing. The dynamics of the examined system is described by stochastic differential equations. We consider an optimal control problem with quadratic costs functional for the linearized model of a well-stirred bioreactor. The optimal control is based on the optimal robust state estimates. The approximate optimal solution...

This thesis focuses on the design and characterisation of miniature bioreactors and evaluates their potential as a scale-down device for microbial cultivation processes. Miniature bioreactors, such as the one detailed in this work, have been developed by many research groups and companies, and seek to increase throughput at the early stages of bioprocess development. Power input was measured in...

The aim of this paper is to propose a new Kalman Filter Recurrent Neural Network (KFRNN) topology and a recursive Levenberg-Marquardt (L-M) algorithm of its learning capable to estimate states and parameters of a highly nonlinear Continuous Stirred Tank Bioreactor (CSTR) in noisy environment. The estimated parameters and states obtained by the proposed KFRNN identifier are used to design an ind...

A stirred tank bioreactor (STB) integrated with an expanded bed adsorption (EBA) system containing anion-exchange resin (Diaion WA30) was developed for in situ removal of acetate to increase the production of α-interferon-2b (α-PrIFN-2b) by Escherichia coli (E. coli). Although the total acetate (9.79 g/L) secreted by E. coli in the integrated STB/EBA system was higher than that in a bioreactor ...

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was 1.9...

Adeno-associated virus vectors (AAV) are reported to have a great potential for gene therapy, however, major bottleneck this kind of therapy is the limitation production capacity. Higher specific AAV vector yield often adherent cell systems compared cells in suspension, and microcarrier-based culture well established anchored on larger scale. The purpose present study was explore how microcarri...

Driven by global competition and rising cost pressure in the pharmaceutical industry, over the last ten years single-use bioreactors have been increasingly used for animal cell cultivations in screening experiments, seed inoculum and seed train productions as well as in small and medium scale production processes of proteins (in particular, antibodies and vaccines). In contrast to re-usable bio...

Human mesenchymal stem cells (hMSCs) are manufactured as cell therapy products. This requires a tightly controlled and fully understood production process, usually taking place in stirred-tank bioreactor. In this review, the impact of bioreactor geometry well equipment-related parameters is discussed. We provide theoretical background for engineering hMSC spheroids with defined size evaluate pr...

The modified rotating simplex method has been successfully used to determine the best combination of agitation rate and aeration rate for maximum production of extracellular proteases by Staphylococcus aureus mutant RC128, in a stirred tank bioreactor operated in a discontinuous way. This mutant has shown altered exoprotein production, specially enhanced protease production. Maximum production ...