It has been found that appropriate probiotic applications increased growth performance and disease resistance in shrimp. Bacillus subtilis has been suggested as a potent probiotic in improving growth performance and enhancing immune response in white shrimp, Litopenaeus vannamei. The aim of this work was to evaluate the possible effect of B. subtilis administration on the meat fatty acid profil...

It has been found that appropriate probiotic applications increased growth performance and disease resistance in shrimp. Bacillus subtilis has been suggested as a potent probiotic in improving growth performance and enhancing immune response in white shrimp, Litopenaeus vannamei. The aim of this work was to evaluate the possible effect of B. subtilis administration on the meat fatty acid profil...

Although several strains of B. subtilis with antifungal activity have been isolated worldwide, to date there are no published reports regarding the isolation of a native B. subtilis strain from strawberry plants in Mexico. A native bacterium (Bacillus subtilis 21) demonstrated in vitro antagonistic activity against different plant pathogenic fungi. Under greenhouse conditions, it was shown that...

The Escherichia coli lep gene, encoding signal peptidase I (SPase I) was provided with Bacillus subtilis transcription/translation signals and expressed in this organism. When present on a low-copy-number plasmid, the amount of E. coli SPase I produced (per mg cell protein) in B. subtilis was half that produced in wild-type E. coli cells. The production of E. coli SPase I in B. subtilis was inc...

Translocation, processing and secretion of YvaY, a Bacillus subtilis protein of unknown function, were characterised both in B. subtilis and in Escherichia coli. In its natural host B. subtilis, YvaY was transiently synthesised at the end of the exponential growth phase. It was efficiently secreted into the culture supernatant in spite of a calculated membrane spanning domain in the mature part...

background: one of the most important producers of high quality industrial enzymes is the gram-positive bacterium, bacillus subtilis (b. subtilis). one major limitation that hinders the wide application of b. subtilis is the secretion of high levels of extracellular proteases which degrade the secreted foreign proteins. in this study, homologus recombination technique was used to knock out its ...

Bacillus subtilis (B. subtilis) is a potential and attractive host for the production of recombinant proteins. Different expression systems for B. subtilis have been developed recently, and various target proteins have been recombinantly synthesized and purified using this host. In this chapter, we introduce a generic protocol to express a recombinant protein in B. subtilis. It includes protoco...

The chemotaxis machinery of Bacillus subtilis is similar to that of the well characterized system of Escherichia coli. However, B. subtilis contains several chemotaxis genes not found in the E. coli genome, such as cheC and cheD, indicating that the B. subtilis chemotactic system is more complex. In B. subtilis, CheD is required for chemotaxis; the cheD mutant displays a tumbly phenotype, has a...

sesame oil cake (ssc) was bio-processed through solid state fermentation (ssf) under optimized conditions by a phytase-producing fish gut bacterium, bacillus subtilis subsp. subtilis (jx292128). ssf significantly reduced anti-nutritional factors (e.g., phytic acid, tannins and trypsin inhibitor) and crude fibre, while enhanced free amino acids, fatty acids and different minerals. phytase product...