A method for monitoring in vitro polyketide synthesis has been developed whereby nonchromophoric polyketide products are made brightly fluorescent in a simple, rapid, inexpensive, and bioorthogonal manner through CuAAC with a sulforhodamine B azide derivative.

In a novel application of two-photon scanning fluorescence microscopy, three-dimensional spatial distributions of the hydrophilic and hydrophobic fluorescent probes, sulforhodamine B and rhodamine B hexyl ester, in excised full-thickness human cadaver skin were visualized and quantified. Both sulforhodamine B and rhodamine B hexyl ester were observed to lie primarily in the lipid multilamellae ...

Four sorbicillinoids (1-4) and seven bisorbicillinoids (5-11), including two new compounds, 6-demethylsorbicillin (1) and 10,11-dihydrobisvertinolone (6), were isolated from a marine-derived fungus Trichoderma sp. Their cytotoxic activities against HL-60 cell line were evaluated by Sulforhodamine B (SRB) assay method and flow cytometric analysis.

Atranorin 1, Usnic acid 2, and D-Arabinitol 3 were isolated from Usnea aciculifera by extensive column chromatography. The structures elucidated based on the comprehensive spectral analysis including FT IR, GC MS, ESI 13C NMR, 1H NMR. compounds evaluated for their cytotoxic activity against LN-229 glioblastoma cancer cell line performing Sulforhodamine B assay (SRB). 2 showed strong with an IC5...