In the present study we used whole-cell patch clamp recordings to investigate swelling-activated Cl-currents (ICl-swell) in M-1 mouse cortical collecting duct (CCD) cells. Hypotonic cell swelling reversibly increased the whole-cell Cl- conductance by about 30-fold. The I-V relationship was outwardly-rectifying and ICl-swell displayed a characteristic voltage-dependence with relatively fast inac...

In the context of sustainable development which is a balancing act between the fulfilment of human needs and the protection of the natural environment, the use of natural fibers such as coir in geotechnical applications is desirable. Reinforcing the soil with coir fibers/coir geotextiles is a cost effective solution to the ground/soil improvement problems. The effects of fiber parameters on the...

Data acquired from the hyperspectral airborne sensor DAIS-7915 over Antequera in southern Spain was processed to yield a quantitative soil swelling potential map based on three physicochemical soil properties currently used in engineering as measures of soil swelling namely cation exchange capacity (CEC), coefficient of linear extensibility (COLE), and Saturated moisture content (SP). The metho...

AIMS We assessed regulation of volume-sensitive Cl(-) current (I(Cl,swell)) by endothelin-1 (ET-1) and characterized the signalling pathway responsible for its activation in rabbit atrial and ventricular myocytes. METHODS AND RESULTS ET-1 elicited I(Cl,swell) under isosmotic conditions. Outwardly rectified Cl(-) current was blocked by the I(Cl,swell)-selective inhibitor DCPIB or osmotic shrin...

In this investigation, three different swell systems known in pipe organs, the swell box, the crescendo wheel, and the historic wind swell were measured and compared to each other. The dynamic range of the crescendo wheel was found to be most effective, and for frequencies near 2 kHz the increase in sound pressure level could be up to 50 dB between the softest and the loudest adjustment. The ma...

The role of swelling-activated currents in cell volume regulation is unclear. Currents elicited by swelling rabbit ventricular myocytes in solutions with 0.6-0.9x normal osmolarity were studied using amphotericin perforated patch clamp techniques, and cell volume was examined concurrently by digital video microscopy. Graded swelling caused graded activation of an inwardly rectifying, time-indep...

Extrudate swell is often observed to be weakened in nanocomposites compared to the pure polymer matrix. A theory quantifying this would be significant either for optimum processing or for understanding their viscoelasticity. A unified extrudate swell correlation with material properties and capillary parameters was suggested for polymer melt and their nanocomposites when considering the reservo...

When cells swell in hypo-osmotic solutions, chloride-selective ion channels (Cl(swell)) activate to reduce intracellular osmolality and prevent catastrophic cell rupture. Despite intensive efforts to assign a molecular identity to the mammalian Cl(swell) channel, it remains unknown. In an unbiased genome-wide RNA interference (RNAi) screen of Drosophila cells stably expressing an anion-sensitiv...