Fungicides viz., Benomyl, TopsinM, Carbendazim and Cuprocaffro were used at different concentrations i.e., 0, 1, 10, 100, 1000 and 10,000 ppm a.i. to evaluate Trichoderma species viz. T. harzianum, T. pseudokoningii, T. longibrachiatum, T. viride for tolerance to fungicides. Topsin-M and Carbendazim were the most effective fungicides that inhibit the growth of Trichoderma species even at low co...

Protoplasts were isolated from Trichoderma harzianum strain PTh18 using lysing enzymes and self-fusion of T. harzianum protoplasts was carried out using polyethylene glycol in STC buffer. The fused protoplasts of T. harzianum were regenerated and 15 self-fusants were selected to study the chitinase production and biocontrol activity. High chitinase activity was measured in the culture filtrates...

The most common biological control agents (BCAs) of the genus Trichoderma have been reported to be strains of Trichoderma virens, T. harzianum, and T. viride. Since Trichoderma BCAs use different mechanisms of biocontrol, it is very important to explore the synergistic effects expressed by different genotypes for their practical use in agriculture. Characterization of 16 biocontrol strains, pre...

Phylloplane fungi of Chlorophytum tuberosum were screened for their antagonistic activities against Colletotrichum dematium in vitro. Volatiles produced from the cultures of Trichoderma harzianum ISO-1 showed maximum inhibition of mycelial growth of C.dematium followed by T. piluliferum, T. harzianum ISO-2 and Aspergillus niger,

Employing Illumina Hiseq whole genome metagenome sequencing approach, we studied the impact of Trichoderma harzianum on altering the microbial community and its functional dynamics in the rhizhosphere soil of black pepper (Piper nigrum L.). The metagenomic datasets from the rhizosphere with (treatment) and without (control) T. harzianum inoculation were annotated using dual approach, i.e., stan...

The investigation was undertaken to enhance the decomposition process by pre-treatment of rice stubble, having higher concentration of lignin. Air-dried rice stubble was treated with 1.8 liter of 1% NaOH and autoclaved. Six cellulolytic fungi, Trichoderma harzianum, Penicillium citrinum, Curvularia lunata, Aspergillus flavus and Alternaria alternata were grown in basal synthetic medium along wi...

The aim of this study was to develop a protocol for the production of fungal bio-pesticides with high efficiency, low cost, and non-polluting fermentation, while also increasing their survival rate under field conditions. This is the first study to develop biocontrol Trichoderma harzianum transformants TS1 that are resistant to benzimidazole fungicides. Agricultural corn stover and wheat bran w...

Research was carried out to evaluate the effectiveness of the biological control of the Botrytis gray mould, caused by Botrytis cinerea Pers., one of the most important fungal diseases of the tomato (Lycopersicon esculentum Mill.). Biological control was performed by using Trichoderma harzianum Rifai, an antagonist that is a naturally occurring fungus found on some plants and in the soil worldw...

trichoderma harzianum (t39), t.virens (dar74290), t.viride (mo), t. harzianum (m) and trichderminb a commercial formulation of t. harzianum(bi) were evaluated as potential biological agents for the control of charcoal stem rot in melon caused by macrophomina phaseolina. cell-free metabolites of t. harzianum(m), t. harzianum (t39) and t. virens (dar 74290) inhibited growth of m. phaseolina compl...

Although a wide application of pesticides and herbicides are an essential part of augmenting crop yields, excessive use of these chemicals leads to the microbial imbalance of soil, environmental pollution and health hazards to human and animals. An ideal pesticide should have the ability to destroy target pests quickly and should get degrade the toxic and nontoxic substances as quickly as possi...