The recent advent in single-molecule imaging and manipulation methods has made a significant impact on the understanding of molecular mechanisms underlying many essential cellular processes. Single-molecule techniques such as electron microscopy and DNA fiber assays have been employed to study the duplication of genome in eukaryotes. Here, we describe a single-molecule assay that allows replica...

Abstract The influence of pharmaceutical residues and heavy metals on living organisms has received global attention. present study assessed the interactive effect antibiotic in soil, as contaminated food with cadmium (Cd) oxytetracycline (OTC) isopod Porcellio leavis. It was fed fresh plant leaves different concentrations cadmium, Cd+OTC1000 ppm, Cd+OTC2000 ppm Cd+OTC3000 for 4 weeks. changes ...

In contrast to birds and mammals, no information appears to be available on the molecular adaptations for O(2) transport in high-altitude ectothermic vertebrates. We investigated Hb of the aquatic Andean frog Telmatobius peruvianus from 3,800-m altitude as regards isoform differentiation, sensitivity to allosteric cofactors, and primary structures of the alpha- and beta-chains, and we carried o...

Aquaporin protein regulation and redistribution in response to osmotic stress was investigated. Ice plant (Mesembryanthemum crystallinum) McTIP1;2 (McMIPF) mediated water flux when expressed in Xenopus leavis oocytes. Mannitol-induced water imbalance resulted in increased protein amounts in tonoplast fractions and a shift in protein distribution to other membrane fractions, suggesting aquaporin...

This paper attempts to offer a fresh examination of the relationship between Empson’s early career as poet and his later erudite, largely influential, critical output. The theme waste, which dominates Collected Poems, partly accounts for decision abandon poetry, invest interest in complexity ambiguity modernist/postmodernist procedure, way ahead contemporary literary theory at time. waste is fu...

Residues 89-100 of troponin C (C8s-loo) and 96-116 of troponin 1 (196-116) interact with each other in the troponin complex (Dalgarno, D. C., Grand, R. J. A., Levine, B. A. Moir, A,, J. G., Scott, G. M. M., and Perry, S . V. (1982) FEBS Lett. 150, 54-58) and are necessary for the Caa+ sensitivity of actomyosin ATPase (Syska, H., Wilkinson, J. M., Grand, R. J. A., and Perry, S. V. (1976) Bioclre...

This paper seeks to find out how assumptions surrounding the moralization of reading appear in BookTube videos readers inspired by Rory Gilmore, bibliophilic protagonist Warner Bros. comedy-drama series Gilmore Girls. In doing so, it aims illuminate ways which myth “moralization” is used disguise complex relations between class, privilege, and meritocracy, both within Girls without. Building fr...