The aim of the present study was to find the effects of pre and post vitrification taxol treatment on bovineimmature cumulus oocyte complexes (COCs). Bovine ovaries were collected from the local slaughterhousein a container with 30-37°C physiologic saline and supplemented with antibiotics. The grade A aspiratedCOCs were assigned to 6 experimental groups; The 1st group was control (n=100) in whi...

Background: Annually, various types of cancer cause thousands deaths globally, and identifying an appropriate therapeutic option for these disorders is crucial importance. Side effects anticancer drugs can be reduced through the promising strategy combination therapy. Objectives: The present paper has investigated in vitro cytotoxicity Taxol, carboplatin, vinblastine, vincristine alone against ...

Taxol, a plant-derived antitumor agent, stabilizes microtubules. Taxol also elicits cell signals in a manner indistinguishable from bacterial lipopolysaccharide (LPS). LPS-like actions of Taxol are controlled by the lps gene and are independent of binding to the known Taxol target, beta-tubulin. Using biotin-labeled Taxol, avidin-agarose affinity chromatography, and peptide mass fingerprinting,...

Taxol, a natural plant product that enhances the rate and extent of microtubule assembly in vitro and stabilizes microtubules in vitro and in cells, was labeled with tritium by catalytic exchange with (3)H(2)O. The binding of [(3)H]taxol to microtubule protein was studied by a sedimentation assay. Microtubules assembled in the presence of [(3)H]taxol bind drug specifically with an apparent bind...

The effects of taxol on antitubulin immunofluorescent staining pat terns, cellular DNA content, and labeling with [3H|thymidine were meas ured for the taxol-sensitive III (ill and taxol-resistant K562 cell lines after exposures for 0, 4, 12, and 24 h. Taxol caused a relative increase in the fraction of 4C interphase and metaphase cells in both lines although the 4C interphase accumulation was g...

We have successfully used mutagenesis to engineer Taxol (paclitaxel) binding activity in Saccharomyces cerevisiae tubulin. Taxol, a successful antitumor agent, acts by promoting tubulin assembly and stabilizing microtubules. Several structurally diverse antimitotic compounds, including the epothilones, compete with Taxol for binding to mammalian microtubules, suggesting that Taxol and these com...

Maximizing the yield of taxol per tree is important for the best management of the yew resource. Therefore, the possibility of obtaining additional taxol from the wood was explored. Initial reports form our laboratory, in cooperation with the National Cancer Institute, indicated relatively high concentrations of taxol in Pacific yew heartwood. Although these taxol concentrations were only about...

A previous genome-wide screening analysis identified a panel of genes that sensitize the human non-small-cell lung carcinoma cell line NCI-H1155 to taxol. However, whether the identified genes sensitize other cancer cells to taxol has not been examined. Here, we silenced the taxol-sensitizer genes identified (acrbp, atp6v0d2, fgd4, hs6st2, psma6, and tubgcp2) in nine other cancer cell types (in...

Correlation between expression level of a microtubule-associated protein called ensconsin (E-MAP-115) and degree of Taxol sensitivity in several cultured cell lines prompted us to investigate potential cause-and-effect relationships between ensconsin level and Taxol action. We used human MCF-7 or HeLa cells, which are sensitive to low Taxol concentrations (LD(50) of 30-35 and 3.5 nM, respective...

Taxol is the prototype of a new class of microtubule stabilizing agents with promising anticancer activity. Several studies show that taxol mimics the actions of lipopolysaccharide (LPS) on murine macrophages. To investigate the mechanism of taxol-induced macrophage stimulation, we evaluated the ability of Rhodobacter sphaeroides diphosphoryl lipid A (RsDPLA) and SDZ 880.431 to block taxol-indu...