Many antiproliferative G-quadruplexes (G4s) arise from the folding of GT-rich strands. Among these, the Thrombin Binding Aptamer (TBA), as a rare example, adopts a monomolecular well-defined G4 structure. Nevertheless, the potential anticancer properties of TBA are severely hampered by its anticoagulant action and, consequently, no related studies have appeared so far in the literature. We wish...

Here we report investigations, based on circular dichroism, nuclear magnetic resonance spectroscopy, molecular modelling, differential scanning calorimetry and prothrombin time assay, on analogues of the thrombin binding aptamer (TBA) in which individual thymidines were replaced by 5-fluoro-2'-deoxyuridine residues. The whole of the data clearly indicate that all derivatives are able to fold in...

Relationship among malondialdehyde (MDA), 2-thiobarbituric acid (TBA)-reactive substances (TBA-RS), and tocopherols in the oxidation of rapeseed oil was investigated. MDA was determined by a new HPLC method with chemical derivatization. When the oil was heated at 170 degrees C, TBA-RS and MDA increased. The contents of TBA-RS were approximately 1.6 times higher than those of MDA. Correlation be...

Trenbolone acetate (TBA), the active compound in Finaplix implants, is a synthetic steroid that is used for promotion of growth of beef cattle. By itself, TBA exerts only moderate effects on growth, performance and carcass characteristics of steers and heifers. When used in combination with estrogen(E)-containing implants, TBA results in dramatically increased growth, improved feed conversion e...

This report investigates and characterizes the mechanism for the novel reversible inactivation of a T303A mutant of rabbit cytochrome P450 (P450) 2E1 by tert-butyl acetylene (tBA). P450 2E1 T303A was inactivated in a time-, concentration-, and NADPH-dependent manner through the formation of two tBA adducts to the P450 heme. Interestingly, losses in enzymatic activity and in the reduced CO spect...

A strain that efficiently degraded methyl tert-butyl ether (MTBE) was obtained by initial selection on the recalcitrant compound tert-butyl alcohol (TBA). This strain, a gram-positive methylotrophic bacterium identified as Mycobacterium austroafricanum IFP 2012, was also able to degrade tert-amyl methyl ether and tert-amyl alcohol. Ethyl tert-butyl ether was weakly degraded. tert-Butyl formate ...

In cancer bioassays, inhalation, but not drinking water exposure to ethyl tertiary-butyl ether (ETBE), caused liver tumors in male rats, while tertiary-butyl alcohol (TBA), an ETBE metabolite, caused kidney tumors in male rats following exposure via drinking water. To understand the contribution of ETBE and TBA kinetics under varying exposure scenarios to these tumor responses, a physiologicall...

Two experiments were conducted at three locations to determine the correct dosage and carrier for trenbolone acetate (TBA) and estradiol (E2) implants in feedlot steers. In the dose-response experiment, 1,296 steers were allotted to six implant treatments (48 pens per location): control, 140 mg of TBA (140/0), 30 mg of E2 (0/30), 20 mg of TBA + 4 mg of E2(20/4), 80 mg of TBA + 16 mg of E2(80/16...

Tag-Based Authorization (TBA) is a hybrid access control model that combines the ease of use of extensional access control models with the expressivity of logic-based formalisms. The main limitation of TBA is that it lacks support for policy administration. More precisely, it does not allow policy-writers to specify administrative policies that constrain the tags that users can assign, and to v...

Tissue composition and skeletal muscle cathepsin D (EC 3.4.23.5) activity were measured in wether lambs treated with trenbolone acetate (TBA) and oestradiol-17 beta (Oe) in combination and female lambs treated with TBA or zeranol. Muscle and liver protein fractional synthesis rates and plasma leucine flux were measured in the female lambs. Male castrate lambs treated with TBA plus Oe showed inc...