The testis has been identified as the organ in which a large number of tissue-enriched genes are present. However, a large portion of transcripts related to each stage or cell type in the testis still remains unknown. In this study, databases combined with confirmatory measurements were used to investigate testis-enriched genes, localization in the testis, developmental regulation, gene express...

Gene duplications have been broadly implicated in the generation of testis-specific genes. To perform a comprehensive analysis of paralogous testis-biased genes, we characterized the testes transcriptome of Drosophila melanogaster by comparing gene expression in testes vs. ovaries, heads, and gonadectomized males. A number of the identified 399 testis-biased genes code for the known components ...

Honey bees (Apis mellifera) are an important social pollinator, crucial in maintaining ecological balance and biodiversity. The haploid-diploid sex determination system determines their sexes where males haploids formed from unfertilized eggs while females diploids fertilized eggs. To better understand the gonad differentiation mechanism honey bees, here transcriptome difference between bee ova...

objective: animal model studies have shown that msy2 and jhdm2a genes have an important role in spermatogenesis process and fertility of male mice. but the potential role of these genes in human spermatogenesis and fertility is not known yet. therefore, we evaluated expression ratios of these genes in testis tissues of men with normal and impaired spermatogenesis. materials and methods: in this...

Cancer/Testis (CT) genes, normally expressed in germ line cells but also activated in a wide range of cancer types, often encode antigens that are immunogenic in cancer patients, and present potential for use as biomarkers and targets for immunotherapy. Using multiple in silico gene expression analysis technologies, including twice the number of expressed sequence tags used in previous studies,...

The reproductive-related studies, including genetic and genomic such as gonadal transcriptome analyses, have previously focused on the adult spotted scat, with little information juvenile fish. Transcriptomics is a powerful tool that allows for massive parallel analysis to identify differential expression patterns of gene holistically at particular stage in cell or tissue development. This stud...

objective(s):cyclophosphamide (cp) toxicity on testis was hampered by squid ink polysaccharide (sip) via restoration of antioxidant ability in our previous investigations. this study investigated roles of nrf2/are signal pathway in testis of treated mice. materials and methods: male kunming mice were employed to undergo treatment with sip and/or cp. protein levels of nrf2, keap-1, histone deace...

We have developed a new strategy for differential screening of genes that are expressed in two or more tissues, and have used it to identify genes that are preferentially expressed in both testis and ovary. In this approach, testis-specific cDNAs were first generated using the suppression subtractive hybridization technique, cloned and arrayed in microplates. The inserts from the subtracted tes...