نتایج جستجو برای: thawed

تعداد نتایج: 3873  

2015
Kazumi KISHIDA Mitsuhiro SAKASE Kenta MINAMI Miyuki M. ARAI Reiko SYOJI Namiko KOHAMA Takayuki AKIYAMA Akio OKA Hiroshi HARAYAMA Moriyuki FUKUSHIMA

The purposes of this study were to examine the relationship between male artificial insemination (AI) fertility and sperm acrosomal conditions assessed by new and conventional staining techniques and to identify possible reproductive dysfunctions causing low conception rates in AI using frozen-thawed spermatozoa with poor acrosomal conditions in Japanese Black bulls. We investigated individual ...

Journal: :Animal : an international journal of animal bioscience 2008
M I R Khan A Ijaz

In the first experiment, osmotic pressure of semen and seminal plasma in a semen sample from each of the 20 mature Nili-Ravi buffalo bulls was determined. In the second experiment, effects of osmotic pressure on motility (%), plasma membrane integrity (%) and viability (%) in fresh and frozen-thawed semen samples from each of the seven mature Nili-Ravi buffalo bulls was determined. In the first...

2016
Yong Soo Hur Eun Kyung Ryu Seung Hyun Song San Hyun Yoon Kyung Sil Lim Won Don Lee Jin Ho Lim

OBJECTIVE To study the clinical outcomes of single frozen-thawed blastocyst transfer cycles according to the hatching status of frozen-thawed blastocysts. METHODS Frozen-thawed blastocysts were divided into three groups according to their hatching status as follows: less-than-expanded blastocyst (≤EdB), hatching blastocyst (HgB), and hatched blastocyst (HdB). The female age and infertility fa...

2017
Wanqiu Zhao Jun Hao Shuang Wang Mingzhao Li Xiaoli Zhao Xiuliang Feng Juanzi Shi

Objectives: To compare the clinical outcomes of single fresh and frozen-thawed superior blastocyst transfer in patients for the first transfer. Methods: This study included 770 single fresh blastocyst and 639 single frozenthawed blastocyst transfers from January 2013 to January 2016. All the fresh and frozen-thawed embryo transfer was first transfer for the patients. The patients were divided i...

Journal: :Fertility and sterility 2000
E K Steele N McClure S E Lewis

OBJECTIVE To assess the effects of two methods of freezing on testicular sperm DNA from subjects with obstructive azoospermia and to compare these with samples of fresh and freeze-thawed testicular sperm from fertile men. DESIGN The Comet assay was used to determine the percentage of undamaged DNA in fresh testicular sperm, testicular sperm freeze-thawed in suspension and in a biopsy sample (...

Journal: :Applied and environmental microbiology 1988
P S Nassos A D King A E Stafford

Lactic acid concentrations increased in refrigerated and freeze-thawed anaerobically stored ground beef. Bacterial counts were higher in refrigerated samples, but the ratios of gram-positive bacteria in refrigerated and freeze-thawed samples were the same. No differences in appearance or odor between refrigerated and freeze-thawed samples were noted after 2 days of aerobic storage. Initial lact...

2016
J. B. Patel A. J. Dhami

Sixty semen ejaculates from 10 mature bulls, 5 each of Jafarabadi and Mehsana breed, were studied for sperm motility and velocity parameters of fresh and frozen-thawed spermatozoa using computer assisted sperm analyzer (CASA). The mean values of motile and progressively motile spermatozoa observed in fresh semen of Jafarabadi and Mehsana bulls (79.77±1.62 and 61.80±1.85, and 78.90±1.22 and 61.3...

Journal: :Animal reproduction science 2011
A Heise P N Thompson D Gerber

Fresh and post-thaw parameters (motility, morphology and viability) of stallion epididymal spermatozoa that have been and have not been exposed to seminal plasma were evaluated, and directly compared to fresh and post-thaw parameters of ejaculated spermatozoa. Six sperm categories of each stallion (n=4) were evaluated for motility, morphology and viability. These categories were fresh ejaculate...

2017
Kenji Yamatoya Kazuki Saito Takakazu Saito Woojin Kang Akihiro Nakamura Mami Miyado Natsuko Kawano Yoshitaka Miyamoto Akihiro Umezawa Kenji Miyado Hidekazu Saito

Aim To evaluate the use of frozen embryos on the outcome of assisted reproductive technology (ART), a retrospective study of the Japanese Assisted Reproductive Technology Registry data during the years 2007-2012 was conducted. Methods A total of 124 946 singleton neonates who reached term gestation following ART from 2007-2012, with 80 660 achieved through frozen-thawed embryo transfer (ET) a...

Journal: :Human reproduction 2002
Federica Moffa Francesca Comoglio Lewis C Krey James A Grifo Alberto Revelli Marco Massobrio John Zhang

BACKGROUND We assessed the maturational competence and the chromosomal pattern of mouse oocytes reconstructed by germinal vesicle (GV) transfer technique using nuclear and/or cytoplasmic components from cryopreserved GV stage oocytes. METHODS From 657 GV oocytes (326 fresh and 331 frozen/thawed), four groups of reconstructed oocytes were obtained by micromanipulation and electrofusion: fresh ...

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