in this research work, the zinc extraction was investigated, using bioleaching process from a low grade zinc concentrate which was produced from the accumulated flotation tailings. zinc content was initially upgraded to 11.97% by flotation process. bioleaching experiments were designed and carried out by a mixed culture of acidithiobacillus ferrooxidans, acidithiobacillus thiooxidans, leptospir...

Analysis of deoxyribonucleic acid (DNA) from four species of Clostridium, including two thermophiles, a mesophile, and a psychrophile, revealed no obvious relationship between growth temperature and DNA base composition. The melting temperatures (T(m)) of the DNA from the four species varied no more among the thermophilic, mesophilic, and psychrophilic species than among many related mesophilic...

The activity of components of the extracellular cellulase system of the thermophilic fungus Sporotrichum thermophile showed appreciable differences between strains; beta-glucosidase (EC 3.2.1.21) was the most variable component. Although its endoglucanase (EC 3.2.1.4) and exoglucanase (EC 3.2.1.91) activities were markedly lower, S. thermophile degraded cellulose faster than Trichoderma reesei....

The genome sequence of the extremely thermophilic archaeon Methanococcus jannaschii provides a wealth of data on proteins from a thermophile. In this paper, sequences of 115 proteins from M. jannaschii are compared with their homologs from mesophilic Methanococcus species. Although the growth temperatures of the mesophiles are about 50 degrees C below that of M. jannaschii, their genomic G+C co...

The purification and characterization of a new restriction endonuclease, BclI from the extreme thermophile Bacillus caldolyticus is reported. This enzyme recognizes the sequence : formula: (see text) and cleaves at the positions indicated by the arrows.

Here, we present the draft genome sequence of Thermus filiformis strain ATCC 43280, a thermophile bacterium capable of producing glycosylated carotenoids acylated with branched fatty acids and enzymes of biotechnological potential.

In uitro poly(U)-directed polyphenylalanine synthesis, catalyzed by a cell-free extract of an extreme thermophile, Thermus thermophilus, at 65”C, required the presence of a polyamine such as spermine (1). To study the implications of polyamines in protein synthesis in uiuo, the polyamine composition in the cells of the thermophile was investigated. Two polyamines were extracted as major polyami...

This study presents a review on applications of phytase from thermophilic fungal isolate, Sporotrichum thermophile. Under optimization, improvement in phytase production can be achieved in both solid state (2-fold) and submerged (2.6-fold) fermentations. Phytase could also be produced by alginate-immobilized S. thermophile, and enzyme production was sustainable over 5 repeated cycles. Purified ...

Absorbance-temperature profiles reveal that both the 30S and 50S ribosomal subunits from Bacillus stearothermophilus are more thermostable than the comparable Escherichia coli particles. Thermophile ribosomes formed by the reassociation of subunits do not display functional heat stability.

The chemoenzymatic synthesis of feruloylated arabino-oligosaccharides has been achieved, using a feruloyl esterase type C from Sporotrichum thermophile (StFaeC). The structure of the feruloylated products was confirmed by ESI-MS(n).