The fatty acid composition of a thermophilic Bacillus sp. was altered by the addition of isobutyrate, isovalerate, a-methylbutyrate, leucine, and isoleucine to the growth medium. With isobutyrate, 81% of the fatty acids had 16 carbon atoms and 79% were iso-fatty acids with an even number of carbon atoms. With leucine, 58% of the fatty acids had 15 carbon atoms and 86% were iso-fatty acids with ...

BACKGROUND AND OBJECTIVES Thermophilic bacteria are less studied but important group of microorganisms due to their ability to produce industrial enzymes. MATERIALS AND METHODS In this study, thermophilic bacteria were isolated from hot spring of Tarabalo, India. A bacterium that could tolerate high temperatures was characterized by morphology, biochemistry and sequencing of its 16S rRNA gene...

Thermophilic bacteria belonging to Bacillus genetic group 5 have been reclassified as being members of Geobacillus gen. nov., with G. stearothermophilus as the type strain. Geobacillus species, literally meaning earth or soil Bacillus, are widely distributed and readily isolated from natural and man-made thermophilic biotopes. Work within our group has however shown that an abundance of genetic...

† L.W. and Y.C. contributed equally to this study. 17 * Corresponding author. 18 Mailing address: Institute of Microbiology, Chinese Academy of Sciences, Beijing 19 100101, People’s Republic of China 20 E-mail: [email protected] (B. Yu) 21 Phone/Fax: +86-10-64806132 22 AEM Accepts, published online ahead of print on 12 September 2014 Appl. Environ. Microbiol. doi:10.1128/AEM.01864-14 Copyright © 201...

mesophilic bacteria, fungi and actinomycetes varied during composting cycles with high numbers in the initial and final cycles with maximal values in compost c and d, and sharply decreased in the heating cycles. staphylococcus aureus and bacillus subtilis were dominated at the initial composting cycle. whereas, at maturity bacillus subtilis was the major followed by b.badies, b.polymixa and b.b...

The present study aimed to isolate soil microorganisms-degrading plant latices and to screen their capablilities of producing protease and lipase. Many bacterial and fungal strains of mesophilic, thermotolerant or thermophilic nature were able to grow on sugar-free medium amended with crude latex of either Euphorbia pulcherrima, Ficus elastica or F. nitida and incubated at 25oC, 36oC or 45oC. T...

Soil samples with temperatures up to 64 degrees C were collected from Mount Erebus, an active volcano located on Ross Island, Antarctica. Acridine orange direct counts and most probable number counts of soil samples stored at 4 degrees C for 2 months showed a wide variation in the number of thermophilic microorganisms in different soils. Organisms similar to Clostridium thermohydrosulfuricum, B...

It has long been known that amino acid substitutions in proteins of organisms living at moderate and high temperatures (mesophiles and thermophiles, respectively) are not all symmetrical; for example, more aligned sites have lysine in mesophiles and arginine in thermophiles than have the opposite pattern. This is generally taken to indicate that certain amino acids are favored over others by se...

The thermophilic bacterium Bacillus thermoamylovorans produces highly heat-resistant spores that can contaminate food products, leading to their spoilage. Here, we present the whole-genome sequences of four B. thermoamylovorans strains, isolated from milk and acacia gum.

Fourteen thermotolerant and thermophilic bacterial isolates from a hot spring in Guanajuato State, Mexico, were tested for their ability to induce the corrosion of carbon steel in monocultures and, in selected cases, in mixed cultures and co-culture with a sulfate-reducing strain, SRB-M. Characterization by 16S rDNA showed that three of the thermophilic isolates (G9a, G9c, and G11) belong to th...