We confirmed multiple picoeukaryotic algae, Ostreococcus, Micromonas, and Pelagomonas spp., as thiamine (vitamin B1) auxotrophs in laboratory experiments with axenic cultures. Examined strains have half saturation growth constants (Ks) for B1 between 1.26 and 6.22 pmol B1 L , which is higher than reported seawater concentrations. Minimum B1 cell quotas for Ostreococcus and Micromonas spp. are h...

Vitamin B1 (aneurin, thiamine) is a water-soluble vitamin necessary for the normal function of nervous system, visual system and heart part important enzymes in body. Thiamine enables use glucose, other carbohydrates proteins, supply energy to organism. The main sources thiamine are exogenous small amounts synthesized by microorganisms human intestinal microbiome. cannot accumulate body, so sig...

This technical note describes kinetic fluorescence as an analytical technique to quantify non-fluorescent species. The technique is applied to the determination of thiamine (vitamin B1) in solution. Fluorescence intensity is monitored as thiamine converts to fluorescent thiochrome, and a relationship is established between the rate of increase of intensity and the concentration of thiamine. The...

This technical note describes kinetic fluorescence as an analytical technique to quantify non-fluorescent species. The technique is applied to the determination of thiamine (vitamin B1) in solution. Fluorescence intensity is monitored as thiamine converts to fluorescent thiochrome, and a relationship is established between the rate of increase of intensity and the concentration of thiamine. The...

Thiamine (vitamin B1) is required in the diet of animals, and thiamine deficiency leads to diseases such as beri-beri and the Wernicke-Korsakoff syndrome. Dietary thiamine (vitamin B1) consists mainly of thiamine pyrophosphate (TPP), which is transformed into thiamine by gastrointestinal phosphatases before absorption. It is believed that TPP itself cannot be transported across plasma membranes...

1. The intestinal absorption of [35S]thiamine hydrochloride was investigated in healthy subjects, malnourished alcoholics and a patient with resection of the jejunum and ileum. Serum and urinary radioactivity was studied after administration of 10 pCi of [35S]thiamine hydrochloride in 1-50 mg of non-radioactive thiamine hydrochloride. 2. Results suggest that intestinal absorption of thiamine hy...

In 1953, the authors (1) reported that a decrease of thiamine and vitamin B12 was found in the blood as the result of the energy metabolism of cattle, and that the deficiency of the vitamins was com pletely protected against by a prophylactic injection of the vitamins at the beginning of the experiment. The intimate relation between both vitamins and metabolism was thus recognized for the forma...

Vitamin B1 (C12H67ClN4OS.HCl, molecular weight = 337.33 g mol–1), also called thiamine, has great importance in the food industry in general. Thiamine is a vitamin belonging to the B complex, and can be found in the cuticle of rice, brewer’s yeast, grains, egg yolk, liver, kidney, pork, fish, peanut, walnut, legumes, as well asin green and leafy vegetables. It acts in different parts of the bod...

Thiamine or Vitamin B1 is an essential cofactor for many metabolic processes in numerous tissues. Thiamine-Responsive Megaloblastic Anemia Syndrome is a genetic disorder affecting thiamine transportation with consequent bone marrow, pancreatic, neurological, and cardiac functional and developmental anomalies. There are limited reports of the cardiac manifestations of this syndrome. Here, we pre...