Several liquid-liquid extraction systems containing vanadium {vanadium(IV) or vanadium(V)}, azoderivative of resorcinol {ADR: 4-(2-pyridylazo)-resorcinol (PAR) or 4-(2-thiazolylazo)-resorcinol (TAR)} and (phenylmethoxibis)tetrazolium salts {MBT: 3,3'-(3,3'-dimetoxy-4,4'-biphenylene)-bis(2,5diphenyl-2H-tetrazolium) chloride (Blue Tetrazolium, BT) or 3,3'-(3,3'-dimetoxy-4,4'-biphenylene)bis[2,5-d...

This new assay procedure for diaphorase eliminates problems of high blank rates and nonlinear kinetics associated with other methods. The dye thiazolyl blue tetrazolium bromide is reduced in the presence of NADH and diaphorase to yield a colored formazan, which as maximum absorbance at 560 nm.

DYAn [I] used blue tetrazolium to demonstrate intracellular particles exhibiting marked reducing activity in illuminated and in non-illuminated plant tissues. The blue-black reduced tetrazolium appeared in granules dispersed throughout the cytoplasm of blue-green algae and within the intracellular chloroplasts of green algae and higher plants. Since the dye was reduced more rapidly and at more ...

Leucocyte function was evaluated by the in vivo mobilisation to skin windows with chambers and by the chemotactic, phagocytic, and nitro blue tetrazolium reducing capacity of blood leucocytes from 20 patients with ulcerative colitis. The total number of leucocytes mobilised to the chambers after 12 hours did not differ from those in 21 healthy volunteers. After 24 and 48 hours reduced number of...

Leucocyte function was evaluated by the in vivo mobilisation to skin windows with chambers and by the chemotactic, phagocytic, and nitro blue tetrazolium reducing capacity of blood leucocytes from 20 patients with ulcerative colitis. The total number of leucocytes mobilised to the chambers after 12 hours did not differ from those in 21 healthy volunteers. After 24 and 48 hours reduced number of...