Natural aquatic and soil samples were screened for the presence of thiocyanate-degrading bacteria. Using thiocyanate supplementation, we established an enrichment culture containing such bacteria from lake water. The dominant bacteria had the scnC-LS5 gene encoding thiocyanate hydrolase, which was closely related to the enzyme found previously in Thiobacillus thioparus THI115 isolated from acti...

a factorial experiment based on complete block design was conducted to study the interactive effects of sulfur fertilizer and thiobacillus bacteria on corn growth and root colonization with two species of mycorrhiza fungi. treatments were five levels of sulfur (s0: 0, s1: 50, s2: 100, s3: 150 and s4: 200 mg.kg-1 of dry soil), thiobacillus in two levels [no application (t0) and application of ba...

The presence of thioredoxin was demonstrated in 20 strains of cyanobacteria as well as in one phototrophic bacterium Rhodopseudomonas sulfidophila and in Thiobacillus denitrificans. Thioredoxin activity was not found in Cyanophora paradoxa and in Porphyridium cruentum using the thioredoxin-dependent PAPS-sulfotransferase activity from Synechococcus 6301 as assay system.

Five strains of mesophilic, facultatively organotrophic, ore-leaching eubacteria were isolated from solfatara fields in Iceland and a uranium mine in the Federal Republic of Germany. The new organisms are aerobic gram-negative rods. They can use sulfidic ores or elemental sulfur as sole energy source, indicating that they belong to the genus Thiobacillus. Alternatively, they grow on organic sub...

Rod-shaped bacteria capable of oxidizing ferrous iron at 55 degrees C were cultured from samples of a copper mine leach dump. Yeast extract or cysteine was required by these Thiobacillus-like bacteria for growth, using ferrous iron as an energy source.

An artificial pyritic coal spoil was prepared and examined over a period of 1.5 years for changes in the population of various physiological varieties of bacteria and also for mineral leaching. Heterotrophic bacteria were the first to dominate the spoil, acquiring a population of 10(7) cells per g within 2 weeks. Bacteria capable of utilizing choline sulfate as the sole source of energy compris...

Mercury (Hg) is a toxic heavy metal that poses significant environmental and human health risks. Soils and sediments, where Hg can exist as the Hg sulfide mineral metacinnabar (β-HgS), represent major Hg reservoirs in aquatic environments. Metacinnabar has historically been considered a sink for Hg in all but severely acidic environments, and thus disregarded as a potential source of Hg back to...

The contribution of CO2 to cell material synthesis in Thiobacillus novellus under nutrient-limited conditions was estimated by comparing 14CO2 uptake rates of steady-state autotrophic cultures with that of heterotrophic and mixotrophic cultures at a given dilution rate. Under heterotrophic conditions, some 13% of the cell carbon was derived from CO2; this is similar to the usual anaplerotic CO2...

The nutrient elements uptake in many of soils, is a problem because of high pH and plenty of calcium ions in them. Some of these elements are phosphorus and microelements. It is possible to increase absorption of these nutrient elements by addition of sulfur in soil and biological oxidation. The rate of this reaction is very slow and Thiobacillus bacteria are the most important oxidators of sul...