A high throughput technique used to determine a part of the nucleotide sequence an organism’s genome is called next generation sequencing (NGS). NGS has been Proven revolutionary in genomics. Clinical diagnostics, Plant diseases diagnostic and other aspects medical are now made possible by sequencing. Techniques NGS: there different techniques which being real life sciences i.e., Illumina seque...

High-throughput sequencing (HTS) of citrus indicator hosts, inoculated from psorosis-like diseased samples, revealed the presence first South African variant virus A (CiVA).  This was identified in Italy and is second member Coguvirus genus within order Bunyavirales. The sequence variants CiVA shares 95.55% (RNA1) 94.82% (RNA2) nucleotide identity with Italian sequence. discovery orchards ...

Profiling immune repertoires by high throughput sequencing enhances our understanding of immune system complexity and immune-related diseases in humans. Previously, cloning and Sanger sequencing identified limited numbers of T cell receptor (TCR) nucleotide sequences in rhesus monkeys, thus their full immune repertoire is unknown. We applied multiplex PCR and Illumina high throughput sequencing...

Abstract Two near complete polerovirus genomes were assembled using high throughput sequencing (HTS) data from two separate samples of garlic cultivar ‘Glenlarge’ grown in Gatton, Queensland, Australia. Whole genome sequence comparisons showed that one contig shared 96.7% nucleotide identity with phasey bean mild yellows virus (MT966032.1) and the other, 99.8% turnip (MT586581.1). Phylogenetic ...

TILLING is a method to find mutations in a gene of interest by scanning amplicons from a mutagenized population for sequence changes, commonly a single nucleotide. In the past 5 years, mutation detection by sequencing has become increasingly popular. This chapter details the experimental flow for TILLING-by-Sequencing, highlighting the critical steps involved in tridimensional pooling of genomi...

Sequencing errors and random sampling of nucleotide types among sequencing reads at heterozygous sites present challenges for accurate, unbiased inference of single-nucleotide polymorphism genotypes from high-throughput sequence data. Here, we develop a maximum-likelihood approach to estimate the frequency distribution of the number of alleles in a sample of individuals (the site frequency spec...

Abstract RNA molecules play important roles in almost every cellular process, and their functions are mediated by sequence structure. Determining the secondary structure of RNAs is central to understanding function evolution. probing techniques coupled high-throughput sequencing allow determining structural features at transcriptome-wide scales. Our group recently developed a novel Illumina-bas...