BACKGROUND Helicobacter pylori, which is associated with many upper gastrointestinal diseases, is found in half of the population of the world. Several special stains and immunohistochemistry stain for H. pylori are available. The need for and usefulness of immunohistochemical (IHC) technique has been debated for years. Toluidine blue is a simple stain for microbiological studies and is easily ...

a simple, rapid and sensitive method has been developed for determination of trances of tellurium (vi) (0.096-1.250 mg/ml) based on its catalytic effect on the reduction reaction of toluidine blue by sulfide ion at ph 4. the reaction is monitored spectrophotometrically by measuring the decrease in absorbance of the dyestuff at 628 nm by the fixed time method. the detection limit is 80 ng/ml and...

Toluidine blue stain is used as a marker to differentiate lesions at high risk of progression in order to improve early diagnosis of oropharyngeal carcinomas. This study focused on 45 oral mucosal lesions in 32 patients (13 female, 19 male). In 9 cases, multiple biopsies were collected. Of the 45 lesions examined, 26 (57.0%) were defined clinically benign, while 19 (42.3%) were defined as suspe...

BACKGROUND Hematoxylin and eosin (H&E) is not an optimal stain to discriminate chief cells from parietal cells in gastric biopsies MATERIALS AND METHODS Fifteen sets of biopsies from the gastric corpus were consecutively stained with H&E and toluidine blue stains; chief cells stained deep blue with toluidine blue, thus contrasting with lightly-stained parietal cells. In well-oriented sections...

OBJECTIVE The aim of this study was to assess the importance of early diagnosis of potentially malignant lesions in the prevention of oral cancer. MATERIAL AND METHODS In order to achieve the set objectives, we selected our participant pool. Of the 340 patients who came to the initial examination at the Clinic for Oral Medicine and Periodontology, we selected 40 patients with potentially mali...

Malaria, a febrile illness mostly confined to the tropical countries is transmitted by bite of infected female Anopheles mosquito. In 2015 alone, 88% of the malaria burden and 90% deaths due to malaria were confined to the African and Asian countries. Although number of tests are available for rapid diagnosis and screening for malaria, peripheral blood smear examination remains the gold standar...

Studies were carried out on the photodynamic inactivation of Bacillus subtilis cells by He-Ne laser irradiation in presence of methylene blue and toluidine blue. Electron paramagnetic resonance (EPR) measurements show decrease in the cell membrane nuidity due to photodynamic damage of the lipophilic compartments. Study of cell damage in presence of sodium azide, 0 20 and glutathione as well as ...

Our improved toluidine blue-DNA agar was compared with methyl green DNase test agar for the detection of DNA hydrolysis by campylobacters. The toluidine blue-DNA agar gave clear-cut positive and negative reactions with campylobacter strains belonging to several species.

OBJECTIVE Oral cancer is a significant threat to public health all over the world, especially in Southeast Asia. At the present time, screening of oral cancer, its premalignant stages as well as its early detection, is still largely based on visual examination of the mouth. Visual examination is highly subjective and hence lacks the specificity and sensitivity. The objective of this study was t...