background: rna interference (rnai) is a phenomenon uses double-stranded rna (dsrna) to specifically inhibit gene expression. the non-specific silencing caused by interferon response to dsrna in mammalian cells limits the potential of utilizing rnai to study gene function. duplexes of 21-nucleotide short interfering dsrna (sirna) inhibit gene expression by rnai. in some organisms, sirna can als...

The extraction of intact RNA from oocyte is quite challenging and time-consuming. A standard protocol using commercial RNA extraction kit, yields a low quantity of RNA in oocytes. In the past, several attempts in getting RNA for gene expression study ended up with a few different modified methods. Extraction of high-quality RNA from oocyte is important before further downstream analyses such as...

The rapid progress of RNA-seq technology requires the development of simple, versatile tools that competently reflect RNA levels in the cell. We have developed the TotalScriptTM RNA-Seq Kit, a new, transposome-based directional library prep method suitable for a variety of RNA sample types and a range of input. TotalScript libraries exhibit high complexity, ~97–99% directionality and low rRNA c...