A putative 1079A-->T mutation in the alpha1 isoform of the Na(+), K(+)-ATPase (Atp1a1) gene of the Dahl salt-sensitive rat inbred by John Rapp (SS/Jr) strain was projected to cause a conformation change in the membrane hydrophobic region of the protein product, possibly resulting in hypertension. The existence of the mutation was challenged, but the challenge was apparently rebutted. The New Ze...

Chemical and enzymatic methods were used to synthesize a transition (AT to GC) and a transversion (AT to TA) at a lac operator site known to interact with lac repressor through the thymine 5 methyl group. These operators also contained a poly(dA) . poly(dT) tail 8 to 12 base pairs in length at one end. Results suggest that the steric constraints of lac repressor relative to the position of the ...

A codon-based model for the evolution of protein-coding DNA sequences is presented for use in phylogenetic estimation. A Markov process is used to describe substitutions between codons. Transition/transversion rate bias and codon usage bias are allowed in the model, and selective restraints at the protein level are accommodated using physicochemical distances between the amino acids coded for b...

From a Xgt11 cDNA library derived from isosafrole-treated DBA/2N mouse liver (1), the longest cDNA insert was sequenced by the dideoxy method. Over a range of 1,847 bp, the nucleotide sequence of P2450 is identical to that of P345O cDNA from C5YBL/6N mice (2) except for three isolated changes: C—»G transversion at position 1211, T—J>G transversion at position 1648, and T deleted at position 167...

breast cancer is the most common cancer among women in developed countries. the prevalence of the disease is increasing in the world. its annual incidence among iranian women is about 7000 cases. rap1a, a tumor suppressor gene, is located at 1p13.3 and plays an important role in the cellular adhesion pathway and is involved in the pathogenesis of breast cancer. the dock4 gene, which is located ...