Several papers have already been published for answering the question whether or not the tricarboxylic acid cycle or the C4-dicarboxylic acid cycle plays a role in microorganisms, and a summarizing description of the subject is seen in Ajl's article(1). Karlson and Barker(2), for example, stated that the tricarboxylic acid cycle does not operate in Azotobacter agilis, Barron, Ardao and Hearon (...

The presence of a tricarboxylic acid cycle in Penicillium chry8ogenum, as yet, has not been demonstrated conclusively. Ajl (1951) stated that of the mechanisms proposed for oxidation of the 2 carbon fragment in mold metabolism, the evidence for a tricarboxylic acid cycle is the least convincing. This paper presents a study of some of the enzymes of the tricarboxylic acid cycle in cell-free prep...

Acetobacter suboxydans does not contain an active tricarboxylic acid cycle, yet two pathways have been suggested for glutamate synthesis from acetate catalyzed by cell extracts: a partial tricarboxylic acid cycle following an initial condensation of oxalacetate and acetyl coenzyme A. and the citramalate-mesaconate pathway following an initial condensation of pyruvate and acetyl coenzyme A. To d...

The quantitative significance of the tricarboxylic acid cycle in the oxidation of acetate by yeast has remained in doubt despite many investigations concerning this topic. All of the enzymes of the tricarboxylic acid cycle have been demonstrated in either commercial baker's yeast or in pure cultures of Saccharomyces cerevisiae. (Weiland and Sonderhoff, 1932; Lynen and Neciullah, 1939; Novelli a...

Some aspects of tricarboxylic acid-cycle activity during differentiation and aging in Dictyostelium discoideum were examined. The concentrations of glutamate, aspartate, alanine, citrate, 2-oxoglutarate, succinate, fumarate, malate, oxaloacetate, pyruvate and acetyl-CoA were determined at four stages over the course of differentiation. The rate of 02 utilization was also determined over differe...