Sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa’s lesser uptake of exogenous DNA. In the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton X100 and DMSO, on exogenous DNA uptake by sheep spermatozoa and...

Cell extraction with cold nonionic detergents or alkaline carbonate prepares an insoluble membrane fraction whose buoyant density permits its flotation in discontinuous sucrose gradients. These lipid "rafts" are implicated in protein sorting and are attractive candidates as platforms that coordinate signal transduction pathways with intracellular substrates. Gap junctions form a direct molecula...

A major component of green tea extracts, catechin (-)-Epigallocatechin gallate (EGCg), has been reported to be biologically active and interacting with membranes. A recent study reported drastic effects of EGCg on giant unilamellar vesicles (GUVs). In particular, EGCg above 30 microM caused GUVs to burst. Here we investigated the effect of EGCg on single GUVs at lower concentrations, believing ...

An affinity tag consisting of six adjacent histidine residues followed by an enterokinase cleavage site was genetically engineered at the N-terminus of the glutamate transport protein GltT of the thermophilic bacterium Bacillus stearothermophilus. The fusion protein was expressed in Escherichia coli and shown to transport glutamate. The highest levels of expression were observed in E. coli stra...

In this experimental study, the results of utilizing a nano-lubricant in place compressor work fluid refrigeration system were examined. R134a refrigerant was utilized system. Polyol ester (POE) used as base and prepared from alumina oxide (Al2O3) nanoparticles utilized. The experiments carried out separately with nano-lubricants created by mixing Al2O3 ratios 0.5% 1.0% into POE oil. Additional...

The United States Pharmacopeia (USP) General Chapters Dissolution 〈711〉 and Disintegration and Dissolution of Dietary Supplements 〈2040〉 allows the use of enzymes in dissolution media when gelatin capsules do not conform to dissolution specifications due to cross linking. Possible interactions between enzymes and surfactants when used together in dissolution media could result in loss of the en...

Bu çalışmada, 5-Metil-4-(2-tiazolilazo)-resorsinolün spektroskopik ve elektrokimyasal davranışı üzerindeki yüzey aktif madde etkileri, nötr pH'ta sulu bir çözeltide incelenmiştir. Araştırmada dodesilpiridinyum klorür (DDPC), setilpiridinyum (CPC) (katyonik), Triton X100, Tween 20 (noniyonik) sodyum dodesil sülfat (SDS) (anyonik) kullanılmıştır. Yüzey maddenin eklenmesi üzerine boyarmaddenin abs...

The hypolipidemic activity of Lepidium sativum (family: Brassicaceae) seed sapogenin and flavonoid extract (LSTS and LSTF) was studied against Triton x-100 and high cholesterol diet (HCD) induced hyperlipidemia on rats. The parameters assessed were serum total protein, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDLc), low density lipoprotein cholesterol (LD...

Progress is reported towards the development of a new route to obtain cadmium sulfide (CdS) thin films by using ethylene-diamine-tetra-acetic acid (EDTA) ligand on the chemical bath deposition (CBD) method. Different chemical baths are used to study changes in the structures of the CdS films for different EDTA concentrations. X-ray diffraction (XRD) is used to prove the structural characterizat...

A mixture of benzalkonium chloride, Triton X100, and citric acid (Resiguard F) had a marked virucidal effect on lipid-containing deoxyribonucleic and ribonucleic acid viruses, such as vaccinia virus, herpesvirus, and influenza virus. Adenoviruses and picornaviruses were more resistant to inactivation. Electron microscopy showed that influenza particles became aggregated in the presence of Resig...