Trypsin is a key protease related to digestion and absorption of proteins, which its inhibition must be studied when natural compounds, such as flavonoids, are used part alternative treatments for obesity diabetes mellitus type 2, since trypsin together with other pancreatic enzymes worked at small intestine. Considering that flavonoids good lipase amylase inhibitors, trypsin-flavonoids interac...

Proteolysis of bovine serum albumin by trypsin.

This paper describes smart sampling to be used for bottom-up protein analysis. Four different manners immobilize trypsin on cellulose were evaluated. Untreated paper, potassium-periodate-functionalized (with and without post-immobilization reduction) 2-hydroxyethyl methacrylate (HEMA)/2-vinyl-4,4-dimethylazlactone (VDM)-functionalized all trypsin. For the evaluation, Coomassie Brilliant Blue st...

background: despite the extensive information available in the literature, cell surface marker signature of human amniotic epithelial cells (haecs) remains controversial. the aim of the present study was to characterize immuno-phenotypic features, proliferative capacity and immunogenicity of haecs. we also tested whether expression of some cell surface markers is influenced by the type of tryps...

in this study, the chemical factors effect of metal ions, surfactant, oxidizing agents and enzyme inhibitors on the activity of trypsin and chymotrypsin of rainbow trout fry were considered. the results showed that k+ and na+ had not significant decrease on trypsin and chymotrypsin activity than did those from control (p>0.05). ca2+ and mg2+ showed a significant increase in activity of trypsin ...

Trypsin is a long-known serine protease widely used in biochemical, analytical, biotechnological, or biocatalytic applications. The high biotechnological potential based on its catalytic activity, substrate specificity, and robustness non-physiological reaction conditions. latter mainly due to stable protein fold, which six intramolecular disulfide bridges make significant contribution. Althoug...