Thrombospondin (TSP) is a multidomain adhesive protein postulated to play an important role in the biological activity of the extracellular matrix. To test this hypothesis, TSP-containing fibrin and collagen matrices were evaluated for their capacity to support angiogenesis and cell growth from explants of rat aorta. This serum-free model allowed us to study the angiogenic effect of TSP without...

PURPOSE To investigate the production and release of thrombospondin-1 (TSP-1), a natural inhibitor of angiogenesis, by human retinal pigment epithelial (RPE) cells to clarify the possible role of TSP-1 in maintaining intraocular angiogenesis. METHODS Human RPE cells were isolated from a human cadaveric eye and cultured in medium with 5% newborn calf serum. TSP-1 messages in the purified RNA o...

results the rate of first-time successful spinal needle insertion was not significantly different between the clsp and tsp groups (62.9% versus 55.7%, p > 0.05). ease of landmark palpation in the clsp group was not significantly different from that in the tsp group (86.7% versus 76.4%, p > 0.05). the number of needle-bone contacts in both groups were not significantly different (p > 0.05). the ...

Thrombospondins (TSPs) are multifunctional matricellular glycoproteins which are involved in the regulation of angiogenesis, proliferation, apoptosis, the NO-cGMP-dependent protein kinase pathway and transforming growth factor (TGF) beta activation. The TSP family consists of 5 members, but currently only data on effects of TSP-1 and TSP-2 in renal disease are available. Both TSPs are hardly ex...

Background. Transseptal puncture (TSP) can be challenging. We compared safety and efficacy of a modified TSP technique ("mosquito" technique, MOSQ-TSP) to conventional TSP (CONV-TSP). Method. Patients undergoing AF ablation in whom first attempt of TSP did not result in left atrial (LA) pressure (failure to cross, FTC) were randomized to MOSQ-TSP (i.e., puncture of the fossa via a wafer-thin in...

OBJECTIVE To investigate the role of thrombospondin 1 (TSP-1) in RA. METHODS Expression of TSP-1 in synovial tissues was determined by immunohistochemistry. Expression of TSP-1 in rheumatoid fibroblast-like synovial cells (FLS) was investigated by quantitative real-time PCR and ELISA. Correlations among the plasma TSP-1 and other variables in patients with RA were examined. RESULTS Expressi...

since particulate matter (total suspended particles and particulate matter with median aerodynamic diameter less than 10 µm) is one of the important pollutants in the ambient air of tehran, the capital of iran, organic substances, inorganic substances and lead levels in tsp and pm10, correlation between tsp and pm10 concentrations, ratio among the two fractions and relation between tsp and pm10...

This paper introduces a new notion related to the traveling salesperson problem (TSP) — the notion of the TSP ratio. The TSP ratio of a TSP instance I is the sum of the marginal values of the nodes of I divided by the length of the optimal TSP tour on I, where the marginal value of a node i ∈ I is the difference between the length of the optimal tour on I and the length of the optimal tour on I...

The ability of thrombospondin (TSP), an extracellular matrix glycoprotein, and two proteolytic fragments to support adhesion and neurite outgrowth from embryonic dorsal root ganglia, spinal cord neurons, and PC12 cells was examined. Anti-TSP antibodies or a synthetic peptide (GRGDS) containing an RGD cell-binding region was also added to cells plated on TSP. TSP and its 140 kd fragment were mor...

Previous reports have established that line 1, a spontaneous BALB/c lung carcinoma, expresses a Mr 180,000 tumor-associated surface antigen (TSP-180). In this study, using a monoclonal antibody and flow cytometry to quantify cell surface TSP-180 expression, we found that essentially all cells in a tissue culture-adapted line 1 population express TSP-180, but that the amount of TSP-180 expressed...