By the early 1990s, scientists knew that programmed cell death, or apoptosis, was important for tissue development and homeostasis as well as for cancer progression. Because apoptotic cells activate nucleases to cleave their chromatin, cell death could be followed in vitro by extracting DNA from a population of tissue culture cells and running it out on an agarose gel to look for a characterist...

OBJECTIVES To standardize the TUNEL assay by establishing inter- and intraobserver variability, interassay variability, cutoff values, sensitivity and specificity of the assay, and studying the distribution of the DNA damage in a population of infertile men referred to a clinical andrology laboratory. METHODS Seminal ejaculates from 25 healthy male volunteers (controls) and 194 infertile men ...

Abstract Background The routine semen analysis fails to detect sperm DNA damage which contributes the majority of male factor infertility. Sperm fragmentation test (DFI) measures damage. Blastocyst formation is an important step in IVF ± ICSI. At present, literature lacks any data that correlates DFI and blastocyst formation. Main body abstract We searched MEDLINE other databases till 2020 for ...

Transient receptor potential cation (TRPC) channel proteins are abundantly expressed in brain. However, the functions of these TRPC proteins such as TRPC1 are largely unclear. In this study, we reported that TRPC1 deficiency caused movement disorder as measured by swimming test, modified open field test and sunflower seeds eating test. Immunofluorescent staining showed significant loss of both ...

Sperm chromatin integrity is vital for successful pregnancy and transmission of genetic material to the offspring. We evaluated chromatin integrity in sperm from 60 infertile men and 7 fertile donors comparing the sperm chromatin structure assay (SCSA), TdT-mediated-dUTP nick end labeling (TUNEL), the sperm chromatin dispersion (SCD) test, and acridine orange staining technique (AOT). The TUNEL...

Background: Alzheimer disease is the main cause of dementia in middle-aged and elderly people. Considering the improving effects of creatine supplementation on cognitive performance, this study aimed to determine the effects of creatine supplementation on learning, memory, and apoptosis in an experimental model of Alzheimer’s disease. Methods: Thirty-two male Wistar rats each weighing 250±50...